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PMID: 19841276
Published · ppublish
English
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama.
Proceedings of the National Academy of Sciences of the United States of America
·Vol. 106
·No. 44
·2009-11-03
·Pages 18621-6
Abstract
The assembly of DNA barcode libraries is particularly relevant within species-rich natural communities for which accurate species identifications will enable detailed ecological forensic studies. In addition, well-resolved molecular phylogenies derived from these DNA barcode sequences have the potential to improve investigations of the mechanisms underlying community assembly and functional trait evolution. To date, no studies have effectively applied DNA barcodes sensu strictu in this manner. In this report, we demonstrate that a three-locus DNA barcode when applied to 296 species of woody trees, shrubs, and palms found within the 50-ha Forest Dynamics Plot on Barro Colorado Island (BCI), Panama, resulted in >98% correct identifications. These DNA barcode sequences are also used to reconstruct a robust community phylogeny employing a supermatrix method for 281 of the 296 plant species in the plot. The three-locus barcode data were sufficient to reliably reconstruct evolutionary relationships among the plant taxa in the plot that are congruent with the broadly accepted phylogeny of flowering plants (APG II). Earlier work on the phylogenetic structure of the BCI forest dynamics plot employing less resolved phylogenies reveals significant differences in evolutionary and ecological inferences compared with our data and suggests that unresolved community phylogenies may have increased type I and type II errors. These results illustrate how highly resolved phylogenies based on DNA barcode sequence data will enhance research focused on the interface between community ecology and evolution.
MeSH Terms
Base Sequence
DNA, Plant/genetics
Molecular Sequence Data
Panama
Phylogeny
Sequence Analysis, DNA
Species Specificity
Trees/genetics
Tropical Climate
Chemicals
DNA, Plant
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
Email
Published
2009-11-03
Epub
2009-00-19
Pages
18621-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2763884
Subset
IM
Databases
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