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LiteratureArticle Details
PMID: 15928076
Published · ppublish
English
Journal Article
Research Support, Non-U.S. Gov't
Use of DNA barcodes to identify flowering plants.
Proceedings of the National Academy of Sciences of the United States of America
·Vol. 102
·No. 23
·2005-06-07
·Pages 8369-74
Abstract
Methods for identifying species by using short orthologous DNA sequences, known as "DNA barcodes," have been proposed and initiated to facilitate biodiversity studies, identify juveniles, associate sexes, and enhance forensic analyses. The cytochrome c oxidase 1 sequence, which has been found to be widely applicable in animal barcoding, is not appropriate for most species of plants because of a much slower rate of cytochrome c oxidase 1 gene evolution in higher plants than in animals. We therefore propose the nuclear internal transcribed spacer region and the plastid trnH-psbA intergenic spacer as potentially usable DNA regions for applying barcoding to flowering plants. The internal transcribed spacer is the most commonly sequenced locus used in plant phylogenetic investigations at the species level and shows high levels of interspecific divergence. The trnH-psbA spacer, although short ( approximately 450-bp), is the most variable plastid region in angiosperms and is easily amplified across a broad range of land plants. Comparison of the total plastid genomes of tobacco and deadly nightshade enhanced with trials on widely divergent angiosperm taxa, including closely related species in seven plant families and a group of species sampled from a local flora encompassing 50 plant families (for a total of 99 species, 80 genera, and 53 families), suggest that the sequences in this pair of loci have the potential to discriminate among the largest number of plant species for barcoding purposes.
MeSH Terms
Atropa belladonna/genetics
Cell Nucleus/genetics
DNA, Chloroplast/genetics
DNA, Intergenic/genetics
DNA, Plant/genetics
Electronic Data Processing/methods
Flowers/classification,cytology,genetics
Genes, Plant/genetics
Magnoliopsida/classification,cytology,genetics
Molecular Sequence Data
Phylogeny
Plastids/genetics
Species Specificity
Tobacco/genetics
Chemicals
DNA, Chloroplast
DNA, Intergenic
DNA, Plant
References (33)
33 references, click to expand
-
Ribosomal ITS sequences and plant phylogenetic inference.Mol Phylogenet Evol. 2003 Dec;29(3):417-34 PMID: 14615184
-
Circumscription and phylogeny of the Laurales: evidence from molecular and morphological data.Am J Bot. 1999 Sep;86(9):1301-15 PMID: 10487818
-
Molecular phylogeny of the Magnoliaceae: the biogeography of tropical and temperate disjunctions.Am J Bot. 2001 Dec;88(12):2275-85 PMID: 21669660
-
Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae).Am J Bot. 1997 Aug;84(8):1120 PMID: 21708667
-
DNA barcoding of parasites and invertebrate disease vectors: what you don't know can hurt you.Trends Parasitol. 2003 Dec;19(12):545-6 PMID: 14642760
-
Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator.Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14812-7 PMID: 15465915
-
Explosive invasion of plant mitochondria by a group I intron.Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14244-9 PMID: 9826685
-
Biological identifications through DNA barcodes.Proc Biol Sci. 2003 Feb 7;270(1512):313-21 PMID: 12614582
-
Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation.Am J Bot. 2003 Jun;90(6):931-6 PMID: 21659189
-
Phylogenetics of Cranichideae with emphasis on Spiranthinae (Orchidaceae, Orchidoideae): evidence from plastid and nuclear DNA sequences.Am J Bot. 2003 May;90(5):777-95 PMID: 21659175
-
Novel perspectives in wood certification and forensics: dry wood as a source of DNA.Proc Biol Sci. 2002 May 22;269(1495):1039-46 PMID: 12028761
-
Mitochondrial substitution rates are extraordinarily elevated and variable in a genus of flowering plants.Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17741-6 PMID: 15598738
-
Now is the time.Philos Trans R Soc Lond B Biol Sci. 2004 Apr 29;359(1444):731-2 PMID: 15253359
-
Historical biogeography and the origin of stomatal distributions in Banksia and Dryandra (Proteaceae) based on their cpDNA phylogeny.Am J Bot. 2002 Aug;89(8):1311-23 PMID: 21665734
-
Interim and archival preservation of plant specimens in alcohols for DNA studies.Biotechniques. 1996 Apr;20(4):657-60 PMID: 8800686
-
Molecular phylogeny of Vincetoxicum (Apocynaceae-Asclepiadoideae) based on the nucleotide sequences of cpDNA and nrDNA.Mol Phylogenet Evol. 2004 May;31(2):689-700 PMID: 15062803
-
Ecosystem rooting depth determined with caves and DNA.Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11387-92 PMID: 10500186
-
Deep-level diagnostic value of the rDNA-ITS region.Mol Biol Evol. 1996 Nov;13(9):1276-95 PMID: 8896380
-
Patterns and relative rates of nucleotide and insertion/deletion evolution at six chloroplast intergenic regions in new world species of the Lecythidaceae.Mol Biol Evol. 2003 Oct;20(10):1710-21 PMID: 12832633
-
A 5.8S nuclear ribosomal RNA gene sequence database: applications to ecology and evolution.Mol Ecol. 1998 Jul;7(7):919-23 PMID: 9691493
-
Evolution of mitochondrial gene content: gene loss and transfer to the nucleus.Mol Phylogenet Evol. 2003 Dec;29(3):380-95 PMID: 14615181
-
The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.EMBO J. 1986 Sep;5(9):2043-2049 PMID: 16453699
-
A molecular phylogeny of the endemic Australian genus Gastrolobium (Fabaceae: Mirbelieae) and allied genera using chloroplast and nuclear markers.Am J Bot. 2001 Sep;88(9):1675-87 PMID: 21669702
-
The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis.Am J Bot. 2005 Jan;92(1):142-66 PMID: 21652394
-
The use of chloroplast DNA to resolve plant phylogenies: noncoding versus rbcL sequences.Mol Biol Evol. 1994 Sep;11(5):769-77 PMID: 7968490
-
Molecular coproscopy: dung and diet of the extinct ground sloth Nothrotheriops shastensis.Science. 1998 Jul 17;281(5375):402-6 PMID: 9665881
-
Identification of Birds through DNA Barcodes.PLoS Biol. 2004 Oct;2(10):e312 PMID: 15455034
-
A molecular analysis of ground sloth diet through the last glaciation.Mol Ecol. 2000 Dec;9(12):1975-84 PMID: 11123610
-
Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species.Proc Biol Sci. 2003 Aug 7;270 Suppl 1:S96-9 PMID: 12952648
-
The plastid chromosome of Atropa belladonna and its comparison with that of Nicotiana tabacum: the role of RNA editing in generating divergence in the process of plant speciation.Mol Biol Evol. 2002 Sep;19(9):1602-12 PMID: 12200487
-
Perspectives on archaeal diversity, thermophily and monophyly from environmental rRNA sequences.Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9188-93 PMID: 8799176
-
The promise of a DNA taxonomy.Philos Trans R Soc Lond B Biol Sci. 2004 Apr 29;359(1444):669-79 PMID: 15253352
-
Bamboozled again! Inadvertent isolation of fungal rDNA sequences from bamboos (Poaceae: Bambusoideae).Mol Phylogenet Evol. 1997 Oct;8(2):205-17 PMID: 9299225
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Email
Published
2005-06-07
Epub
2005-00-31
Pages
8369-74
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1142120
Subset
IM
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