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PMID: 19767417 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The completion of the Mammalian Gene Collection (MGC).

Genome research ·Vol. 19 ·No. 12 ·2009-12-00 ·Pages 2324-33

MGC Project Team, Temple G, Gerhard DS, Rasooly R, Feingold EA, Good PJ, Robinson C, Mandich A, Derge JG, Lewis J, Shoaf D, Collins FS, Jang W, Wagner L, Shenmen CM, Misquitta L, Schaefer CF, Buetow KH, Bonner TI, Yankie L, Ward M, Phan L, Astashyn A, Brown G, Farrell C, Hart J, Landrum M, Maidak BL, Murphy M, Murphy T, Rajput B, Riddick L, Webb D, Weber J, Wu W, Pruitt KD, Maglott D, Siepel A, Brejova B, Diekhans M, Harte R, Baertsch R, Kent J, Haussler D, Brent M, Langton L, Comstock CL, Stevens M, Wei C, van Baren MJ, Salehi-Ashtiani K, Murray RR, Ghamsari L, Mello E, Lin C, Pennacchio C, Schreiber K, Shapiro N, Marsh A, Pardes E, Moore T, Lebeau A, Muratet M, Simmons B, Kloske D, Sieja S, Hudson J, Sethupathy P, Brownstein M, Bhat N, Lazar J, Jacob H, Gruber CE, Smith MR, McPherson J, Garcia AM, Gunaratne PH, Wu J, Muzny D, Gibbs RA, Young AC, Bouffard GG, Blakesley RW, Mullikin J, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Hirst M, Zeng T, Tse K, Moksa M, Deng M, Ma K, Mah D, Pang J, Taylor G, Chuah E, Deng A, Fichter K, Go A, Lee S, Wang J, Griffith M, Morin R, Moore RA, Mayo M, Munro S, Wagner S, Jones SJ, Holt RA, Marra MA, Lu S, Yang S, Hartigan J, Graf M, Wagner R, Letovksy S, Pulido JC, Robison K, Esposito D, Hartley J, Wall VE, Hopkins RF, Ohara O, Wiemann S

Abstract

Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.

MeSH Terms

Animals Cloning, Molecular/methods Computational Biology/methods DNA/biosynthesis DNA, Complementary/genetics Gene Library Genes/genetics Humans Mammals/genetics Mice National Institutes of Health (U.S.) Rats Reverse Transcriptase Polymerase Chain Reaction United States

Chemicals

DNA, Complementary DNA

Authors & Affiliations

128 authors, click to expand affiliations / ORCID

MGC Project Team

Temple Gary

Gerhard Daniela S

Rasooly Rebekah

Feingold Elise A

Good Peter J

Robinson Cristen

Mandich Allison

Derge Jeffrey G

Lewis Jeanne

Shoaf Debonny

Collins Francis S

Jang Wonhee

Wagner Lukas

Shenmen Carolyn M

Misquitta Leonie

Schaefer Carl F

Buetow Kenneth H

Bonner Tom I

Yankie Linda

Ward Ming

Phan Lon

Astashyn Alex

Brown Garth

Farrell Catherine

Hart Jennifer

Landrum Melissa

Maidak Bonnie L

Murphy Michael

Murphy Terence

Rajput Bhanu

Riddick Lillian

Webb David

Weber Janet

Wu Wendy

Pruitt Kim D

Maglott Donna

Siepel Adam

Brejova Brona

Diekhans Mark

Harte Rachel

Baertsch Robert

Kent Jim

Haussler David

Brent Michael

Langton Laura

Comstock Charles L G

Stevens Michael

Wei Chaochun

van Baren Marijke J

Salehi-Ashtiani Kourosh

Murray Ryan R

Ghamsari Lila

Mello Elizabeth

Lin Chenwei

Pennacchio Christa

Schreiber Kirsten

Shapiro Nicole

Marsh Amber

Pardes Elizabeth

Moore Troy

Lebeau Anita

Muratet Mike

Simmons Blake

Kloske David

Sieja Stephanie

Hudson James

Sethupathy Praveen

Brownstein Michael

Bhat Narayan

Lazar Joseph

Jacob Howard

Gruber Chris E

Smith Mark R

McPherson John

Garcia Angela M

Gunaratne Preethi H

Wu Jiaqian

Muzny Donna

Gibbs Richard A

Young Alice C

Bouffard Gerard G

Blakesley Robert W

Mullikin Jim

Green Eric D

Dickson Mark C

Rodriguez Alex C

Grimwood Jane

Schmutz Jeremy

Myers Richard M

Hirst Martin

Zeng Thomas

Tse Kane

Moksa Michelle

Deng Merinda

Ma Kevin

Mah Diana

Pang Johnson

Taylor Greg

Chuah Eric

Deng Athena

Fichter Keith

Go Anne

Lee Stephanie

Wang Jing

Griffith Malachi

Morin Ryan

Moore Richard A

Mayo Michael

Munro Sarah

Wagner Susan

Jones Steven J M

Holt Robert A

Marra Marco A

Lu Sun

Yang Shuwei

Hartigan James

Graf Marcus

Wagner Ralf

Letovksy Stanley

Pulido Jacqueline C

Robison Keith

Esposito Dominic

Hartley James

Wall Vanessa E

Hopkins Ralph F

Ohara Osamu

Wiemann Stefan

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Article Info

Journal

Genome research

Abbr.

Genome Res

ISSN

1549-5469

DOI

10.1101/gr.095976.109

Published

2009-12-00

Epub

2009-00-18

Pages

2324-33

Language

English

Region

United States

NLM ID

9518021

PMCID

PMC2792178

Subset

Grants

NHGRI NIH HHS · U54 HG003273 · United States

PHS HHS · N01-C0-12400 · United States

External Links

PubMed source DOI: 10.1101/gr.095976.109 PMC Full Text View journal details

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The completion of the Mammalian Gene Collection (MGC).

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