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PMID: 12477932 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 26 ·2002-12-24 ·Pages 16899-903

Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA, Mammalian Gene Collection Program Team

Abstract

The National Institutes of Health Mammalian Gene Collection (MGC) Program is a multiinstitutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and mouse gene. ESTs were generated from libraries enriched for full-length cDNAs and analyzed to identify candidate full-ORF clones, which then were sequenced to high accuracy. The MGC has currently sequenced and verified the full ORF for a nonredundant set of >9,000 human and >6,000 mouse genes. Candidate full-ORF clones for an additional 7,800 human and 3,500 mouse genes also have been identified. All MGC sequences and clones are available without restriction through public databases and clone distribution networks (see http:mgc.nci.nih.gov).

MeSH Terms

Algorithms Animals DNA, Complementary/analysis,chemistry Gene Library Humans Mice Open Reading Frames Sequence Analysis, DNA

Chemicals

DNA, Complementary

Authors & Affiliations

84 authors, click to expand affiliations / ORCID

Strausberg Robert L

National Cancer Institute, Bethessda, MD 20892-2580, USA. rls@nih.gov

Feingold Elise A

Grouse Lynette H

Derge Jeffery G

Klausner Richard D

Collins Francis S

Wagner Lukas

Shenmen Carolyn M

Schuler Gregory D

Altschul Stephen F

Zeeberg Barry

Buetow Kenneth H

Schaefer Carl F

Bhat Narayan K

Hopkins Ralph F

Jordan Heather

Moore Troy

Max Steve I

Wang Jun

Hsieh Florence

Diatchenko Luda

Marusina Kate

Farmer Andrew A

Rubin Gerald M

Hong Ling

Stapleton Mark

Soares M Bento

Bonaldo Maria F

Casavant Tom L

Scheetz Todd E

Brownstein Michael J

Usdin Ted B

Toshiyuki Shiraki

Carninci Piero

Prange Christa

Raha Sam S

Loquellano Naomi A

Peters Garrick J

Abramson Rick D

Mullahy Sara J

Bosak Stephanie A

McEwan Paul J

McKernan Kevin J

Malek Joel A

Gunaratne Preethi H

Richards Stephen

Worley Kim C

Hale Sarah

Garcia Angela M

Gay Laura J

Hulyk Stephen W

Villalon Debbie K

Muzny Donna M

Sodergren Erica J

Lu Xiuhua

Gibbs Richard A

Fahey Jessica

Helton Erin

Ketteman Mark

Madan Anuradha

Rodrigues Stephanie

Sanchez Amy

Whiting Michelle

Madan Anup

Young Alice C

Shevchenko Yuriy

Bouffard Gerard G

Blakesley Robert W

Touchman Jeffrey W

Green Eric D

Dickson Mark C

Rodriguez Alex C

Grimwood Jane

Schmutz Jeremy

Myers Richard M

Butterfield Yaron S N

Krzywinski Martin I

Skalska Ursula

Smailus Duane E

Schnerch Angelique

Schein Jacqueline E

Jones Steven J M

Marra Marco A

Mammalian Gene Collection Program Team

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.242603899

rls@nih.gov

Published

2002-12-24

Epub

2002-00-11

Pages

16899-903

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC139241

Subset

External Links

PubMed source DOI: 10.1073/pnas.242603899 PMC Full Text View journal details

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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.

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