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PMID: 1976461 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reduced oxidative burst responses in monocytes and monocyte-derived macrophages from HIV-infected subjects.

Clinical and experimental immunology ·Vol. 82 ·No. 1 ·1990-10-00 ·Pages 10-5

Müller F, Rollag H, Frøland SS

Abstract

Oxidative burst responses of monocytes and monocyte-derived macrophages (MDM) were studied in 40 subjects with HIV infection of different clinical stages. Oxidative burst was assessed as reduction of nitroblue tetrazolium (NBT) with or without stimulants. Results were determined as oxidative burst responses per cell and as a stimulatory ratio between stimulated and unstimulated NBT reduction. Cells from 12 HIV-seronegative homosexual men and 38 blood donors served as control groups. In patients with asymptomatic HIV infection, monocyte oxidative burst responses were reduced compared with the blood donors. In MDM from the same patients, stimulatory ratios were reduced. In AIDS patients, stimulatory ratios of both monocytes and MDM were reduced compared with controls. In contrast to the progressive deterioration of CD4+ lymphocyte counts as well as other immune functions in HIV infection, monocyte oxidative burst responses are impaired already in the asymptomatic phase of the infection, almost to the same extent as in patients with AIDS.

MeSH Terms
Adult CD4-Positive T-Lymphocytes/immunology Cells, Cultured Female Gene Products, gag/blood HIV/immunology HIV Core Protein p24 HIV Infections/immunology Humans Lymphocyte Subsets Macrophages/drug effects,immunology,metabolism Male Monocytes/drug effects,immunology,metabolism Nitroblue Tetrazolium/metabolism Oxygen Consumption/drug effects T-Lymphocytes, Regulatory/immunology Tetradecanoylphorbol Acetate/pharmacology Viral Core Proteins/blood Zymosan/pharmacology
Chemicals
Gene Products, gag HIV Core Protein p24 Viral Core Proteins Nitroblue Tetrazolium Zymosan Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Müller F
Kaptein Wilhelmsen og Frues Institute of Bacteriology, Rikshospitalet, Oslo, Norway.
Rollag H
Frøland S S
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Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
DOI
10.1111/j.1365-2249.1990.tb05396.x
Published
1990-10-00
Pages
10-5
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1535145
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1365-2249.1990.tb05396.x PMC Full Text View journal details
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