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PMID: 1975093 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular mechanisms underlying the expression of the human HOX-5.1 gene.

Nucleic acids research ·Vol. 18 ·No. 15 ·1990-08-11 ·Pages 4361-8

Cianetti L, Di Cristofaro A, Zappavigna V, Bottero L, Boccoli G, Testa U, Russo G, Boncinelli E, Peschle C

Abstract

The complex mechanisms underlying homeobox genes expression involve regulation at transcriptional, post-transcriptional and translational levels. The multiple transcripts of the human HOX-5.1 gene are expressed differentially in tissue- and stage-specific patterns during embryogenesis, and differentially induced by retinoic acid (RA) in human embryonal carcinoma (EC) NT2/D1 cells. We have sequenced 6.3 Kb of the genomic region containing the HOX-5.1 gene and analyzed its mechanisms of expression. Two alternative promoters underlie the transcription of two classes of HOX-5.1-specific mRNAs. These classes differ in tissue and subcellular distribution, induction by RA, structure of the 5'-UT region and mRNA stability: these features are compatible with a differential function of the two classes of transcripts in embryogenesis.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Cell Line Embryo, Mammalian/metabolism Embryonal Carcinoma Stem Cells Gene Expression Regulation Genes, Homeobox Humans Molecular Sequence Data Neoplastic Stem Cells Promoter Regions, Genetic Protein Biosynthesis Restriction Mapping Ribonucleases/metabolism Transcription, Genetic Tretinoin/pharmacology
Chemicals
Tretinoin Ribonucleases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Cianetti L
Department of Hematology and Oncology, Istituto Superiore di Sanità, Rome, Italy.
Di Cristofaro A
Zappavigna V
Bottero L
Boccoli G
Testa U
Russo G
Boncinelli E
Peschle C
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.15.4361
Published
1990-08-11
Pages
4361-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331252
Subset
IM
Databases
GENBANK
X17360
External Links
PubMed source DOI: 10.1093/nar/18.15.4361 PMC Full Text View journal details
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