Home LiteratureArticle Details
PMID: 197275 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetics of resistance to phosphonoacetic acid in strain KOS of herpes simplex virus type 1.

Journal of virology ·Vol. 23 ·No. 3 ·1977-09-00 ·Pages 833-6

Jofre JT, Schaffer PA, Parris DS

Abstract

A DNA- temperature-sensitive mutant of herpes simplex virus type 1 exhibiting thermolabile DNA polymerase activity, tsD9, was shown to be resistant to phosphonoacetic acid (PAA) when plated at the permissive temperature. ts+ revertants of tsD9 were PAA sensitive and exhibited DNA polymerase activity intermediate between that of the wild-type virus and tsD9, indicating that both temperature sensitivity and sensitivity to PAA are controlled by the same gene. Since the position of tsD9 on the existing herpes simplex virus type 1 linkage map is known, the locus for PAA resistance--and therefore for the structural gene for viral DNA polymerase--has been identified.

MeSH Terms
Acetates/pharmacology Antiviral Agents/pharmacology DNA-Directed DNA Polymerase/biosynthesis Drug Resistance, Microbial Genes Genetic Linkage Mutation Organophosphorus Compounds/pharmacology Simplexvirus/drug effects,enzymology Temperature
Chemicals
Acetates Antiviral Agents Organophosphorus Compounds DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jofre J T
Schaffer P A
Parris D S
References (10)
10 references, click to expand
  1. DNA synthesis and DNA polymerase activity of herpes simplex virus type 1 temperature-sensitive mutants.
    J Virol. 1975 Sep;16(3):498-507 PMID: 169388
  2. Isolation of herpes simplex virus clones and drug resistant mutants in microcultures.
    Arch Virol. 1975;49(1):73-80 PMID: 172951
  3. DNA polymerase induction by DNA-negative temperature-sensitive mutants of herpes simplex virus type 2.
    Virology. 1975 Dec;68(2):374-86 PMID: 173076
  4. Mutants of herpes simplex virus types 1 and 2 that are resistant to phosphonoacetic acid induce altered DNA polymerase activities in infected cells.
    J Gen Virol. 1976 Apr;31(1):145-8 PMID: 177727
  5. Herpesvirus proteins: DNA polymerase and pyrimidine deoxynucleoside kinase activities in temperature-sensitive mutants of herpes simplex virus type 2.
    J Gen Virol. 1976 Apr;31(1):65-73 PMID: 177730
  6. Suppression of herpes simplex virus infection by phosphonoacetic acid.
    Appl Microbiol. 1973 Sep;26(3):264-7 PMID: 4356456
  7. Recombination between temperature-sensitive mutants of herpes simplex virus type 1.
    Virology. 1974 Mar;58(1):219-28 PMID: 4362547
  8. Temperature-sensitive mutants of herpes simplex virus type 1: isolation, complementation and partial characterization.
    Virology. 1973 Mar;52(1):57-71 PMID: 4372782
  9. Inhibition of herpes simplex virus replication by phosphonoacetic acid.
    Antimicrob Agents Chemother. 1974 Sep;6(3):360-5 PMID: 15830487
  10. Inhibition of DNA polymerase from herpes simplex virus-infected wi-38 cells by phosphonoacetic Acid.
    J Virol. 1975 May;15(5):1281-3 PMID: 16789161
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.23.3.833-836.1977
Published
1977-09-00
Pages
833-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515897
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.23.3.833-836.1977 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2