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PMID: 1971617 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ultrastructural study of surface components of Streptococcus suis.

Journal of bacteriology ·Vol. 172 ·No. 6 ·1990-06-00 ·Pages 2833-8

Jacques M, Gottschalk M, Foiry B, Higgins R

Abstract

The presence of capsular material on cells of nine reference strains of Streptococcus suis representing serotypes 1 to 8 and 1/2 was determined by transmission electron microscopy after polycationic ferritin labeling, immunostabilization, or fixation with a combination of glutaraldehyde and lysine. All the cells of the reference strains examined were covered with a layer of capsular material whose thickness varied between 20 to 30 nm and 350 to 375 nm when examined by immunostabilization. Capsular material from cells exposed to homologous antiserum was usually thicker than that from polycationic ferritin-labeled cells or cells fixed with glutaraldehyde-lysine. Negative staining revealed detectable surface structures on S. suis strains. All strains carried peritichous, thin, and flexible fimbriae with a diameter of approximately 2 nm and a length of up to 250 nm. This study indicated that morphological differences of surface structure exist among S. suis reference strains.

MeSH Terms
Ferritins/analysis Fimbriae, Bacterial/ultrastructure Microscopy, Electron Streptococcus/ultrastructure
Chemicals
Ferritins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jacques M
Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.
Gottschalk M
Foiry B
Higgins R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.6.2833-2838.1990
Published
1990-06-00
Pages
2833-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209078
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.6.2833-2838.1990 PMC Full Text View journal details
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