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PMID: 1970118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Retinoic acid-induced granulocytic differentiation of HL-60 myeloid leukemia cells is mediated directly through the retinoic acid receptor (RAR-alpha).

Molecular and cellular biology ·Vol. 10 ·No. 5 ·1990-05-00 ·Pages 2154-63

Collins SJ, Robertson KA, Mueller L

Abstract

Retinoic acid (RA) induces terminal granulocytic differentiation of the HL-60 promyelocytic leukemia cell line as well as certain other human myeloid leukemias. Specific RA receptors that are members of the steroid-thyroid hormone superfamily of nuclear transcription factors have recently been identified. We developed an HL-60 subclone that was relatively resistant to RA-induced differentiation. Specific nuclear RA receptors in this RA-resistant subclone had a decreased affinity for RA and exhibited a lower molecular weight compared with nuclear RA receptors from the RA-sensitive parental HL-60 cells. Retroviral vector-mediated transduction of a single copy of the RA receptor (RAR-alpha) into this RA-resistant HL-60 subclone restored the sensitivity of these cells to RA. These observations indicate that RAR-alpha plays a critical and central role in mediating RA-induced terminal differentiation of HL-60 leukemia cells.

MeSH Terms
CD18 Antigens Carrier Proteins/physiology Cell Differentiation/drug effects Cell Division/drug effects Gene Expression Regulation/drug effects Granulocytes/cytology Humans In Vitro Techniques Mutation Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-myc Receptors, Leukocyte-Adhesion/genetics Receptors, Retinoic Acid Transfection Tretinoin/pharmacology Tumor Cells, Cultured
Chemicals
CD18 Antigens Carrier Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc Receptors, Leukocyte-Adhesion Receptors, Retinoic Acid Tretinoin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Collins S J
Molecular Medicine Program, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Robertson K A
Mueller L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.5.2154-2163.1990
Published
1990-05-00
Pages
2154-63
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360563
Subset
IM
Corrections
CommentIn
PMID: 27089249
External Links
PubMed source DOI: 10.1128/mcb.10.5.2154-2163.1990 PMC Full Text View journal details
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