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PMID: 19666572 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Accumulation of ubiquitin conjugates in a polyglutamine disease model occurs without global ubiquitin/proteasome system impairment.

Maynard CJ, Böttcher C, Ortega Z, Smith R, Florea BI, Díaz-Hernández M, Brundin P, Overkleeft HS, Li JY, Lucas JJ, Dantuma NP

Abstract

Aggregation-prone proteins have been suggested to overwhelm and impair the ubiquitin/proteasome system (UPS) in polyglutamine (polyQ) disorders, such as Huntington's disease (HD). Overexpression of an N-terminal fragment of mutant huntingtin (N-mutHtt), an aggregation-prone polyQ protein responsible for HD, obstructs the UPS in cellular models. Furthermore, based on the accumulation of polyubiquitin conjugates in brains of R6/2 mice, which express human N-mutHtt and are one of the most severe polyQ disorder models, it has been proposed that UPS dysfunction is a consistent feature of this pathology, occurring in both in vitro and in vivo models. Here, we have exploited transgenic mice that ubiquitously express a ubiquitin fusion degradation proteasome substrate to directly assess the functionality of the UPS in R6/2 mice or the slower onset R6/1 mice. Although expression of N-mutHtt caused a general inhibition of the UPS in PC12 cells, we did not observe an increase in the levels of proteasome reporter substrate in the brains of R6/2 and R6/1 mice. We show that the increase in ubiquitin conjugates in R6/2 mice can be primarily attributed to an accumulation of large ubiquitin conjugates that are different from the conjugates observed upon UPS inhibition. Together our data show that polyubiquitylated proteins accumulate in R6/2 brain despite a largely operative UPS, and suggest that neurons are able to avoid or compensate for the inhibitory effects of N-mutHtt.

MeSH Terms
Animals Brain/metabolism Disease Models, Animal Green Fluorescent Proteins/metabolism Humans Huntington Disease/diagnosis,genetics Mice Mice, Inbred C57BL Mice, Transgenic Mutation Peptides/genetics,metabolism Proteasome Endopeptidase Complex/metabolism Protein Structure, Tertiary Ubiquitin/chemistry,metabolism
Chemicals
Peptides Ubiquitin Green Fluorescent Proteins polyglutamine Proteasome Endopeptidase Complex
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Maynard Christa J
Department of Cell and Molecular Biology, Karolinska Institutet, S-17177 Stockholm, Sweden.
Böttcher Claudia
Ortega Zaira
Smith Ruben
Florea Bogdan I
Díaz-Hernández Miguel
Brundin Patrik
Overkleeft Hermen S
Li Jia-Yi
Lucas Jose J
Dantuma Nico P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
Published
2009-08-18
Epub
2009-00-30
Pages
13986-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2729007
Subset
IM
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