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PMID: 1966041 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Binding of insulin-like growth factor II (IGF-II) by human cation-independent mannose 6-phosphate receptor/IGF-II receptor expressed in receptor-deficient mouse L cells.

Cell regulation ·Vol. 1 ·No. 2 ·1990-01-00 ·Pages 197-213

Nolan CM, Kyle JW, Watanabe H, Sly WS

Abstract

Mouse L cells deficient in expression of the murine cation-independent mannose 6-phosphate receptor/insulin-like growth factor II receptor (CI-MPR/IGF-IIR) were stably transfected with a plasmid containing the cDNA for the human receptor. Transfected cells expressed high levels of the human receptor which functioned in the transport of lysosomal enzymes and was capable of binding 125I-IGF-II, both at the cell surface and intracellularly. Cell surface binding of 125I-IGF-II by the receptor could be inhibited by pretreatment of cells with antibodies to the receptor or by coincubation with the lysosomal enzyme, beta-glucuronidase. Expression of the receptor conferred on transfected cells the ability to internalize and degrade 125I-IGF-II. Cells transfected with the parental vector and those expressing the human CI-MRP/IGF-IIR were found to express an atypical binding site for IGF-II that was distinct from the CI-MPR/IGF-IIR and the type I IGF-receptor. The availability of two cell lines, one of which overexpresses the human CI-MPR/IGF-IIR and one deficient in expression of the murine receptor, may help in the analysis of the role of the receptor in mediating the biological effects of IGF-II. They should also be useful in examining the significance of binding of ligands, such as transforming growth factor-beta 1 precursor and proliferin to this receptor.

MeSH Terms
Animals DNA/genetics Endocytosis Glucuronidase/pharmacology Humans Insulin-Like Growth Factor II/metabolism L Cells/metabolism Mannosephosphates/metabolism,pharmacology Mice Receptor, IGF Type 2 Receptors, Cell Surface/metabolism Receptors, Somatomedin Recombinant Fusion Proteins/metabolism
Chemicals
Mannosephosphates Receptor, IGF Type 2 Receptors, Cell Surface Receptors, Somatomedin Recombinant Fusion Proteins mannose-6-phosphate Insulin-Like Growth Factor II DNA Glucuronidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nolan C M
Edward A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, Missouri 63104.
Kyle J W
Watanabe H
Sly W S
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Article Info
Journal
Cell regulation
Abbr.
Cell Regul
ISSN
1044-2030
DOI
10.1091/mbc.1.2.197
Published
1990-01-00
Pages
197-213
Language
English
Region
United States
NLM ID
9005331
PMCID
PMC361444
Subset
IM
Grants
NIDDK NIH HHS · DK 40163 · United States
NIGMS NIH HHS · GM 34182 · United States
External Links
PubMed source DOI: 10.1091/mbc.1.2.197 PMC Full Text
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