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PMID: 2828025 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The receptor for insulin-like growth factor II mediates an insulin-like response.

The EMBO journal ·Vol. 6 ·No. 11 ·1987-11-00 ·Pages 3367-71

Hari J, Pierce SB, Morgan DO, Sara V, Smith MC, Roth RA

Abstract

Insulin-like growth factor II (IGF-II) shares sequence homology and predicted three-dimensional structure with insulin and IGF-I. IGF-II can bind, therefore, to a limited extent with the receptors for these two other hormones, as well as to a distinct receptor for IGF-II. Previous studies have been unable to attribute a particular response of IGF-II through its own receptor. In the present studies, the IGF-II receptor is shown to mediate the stimulation of glycogen synthesis in human hepatoma cells since: (i) IGF-II is found to be capable of stimulating a response at concentrations in which it would primarily interact with its own receptor; (ii) the response to IGF-II was not blocked by monoclonal antibodies which inhibit the responses of cells through the insulin and IGF-I receptors; and (iii) polyclonal antibodies to the IGF-II receptor were found to mimic the ability of IGF-II to stimulate glycogen synthesis. These results indicate that the IGF-II receptor mediates a particular biological response--stimulation of glycogen synthesis in hepatoma cells. Furthermore, a monovalent Fab fragment of the polyclonal antibody to the IGF-II receptor was also shown to stimulate glycogen synthesis in these cells. These data indicate that clustering of the IGF-II receptor is not required to stimulate a biological response.

MeSH Terms
Antibodies, Monoclonal Carcinoma, Hepatocellular Cell Line Glycogen/biosynthesis Humans Insulin/immunology,pharmacology Insulin-Like Growth Factor I/pharmacology Insulin-Like Growth Factor II/immunology,pharmacology,physiology Kinetics Liver Neoplasms Receptor, Insulin/physiology Receptors, Somatomedin Somatomedins/pharmacology
Chemicals
Antibodies, Monoclonal Insulin Receptors, Somatomedin Somatomedins Insulin-Like Growth Factor I Insulin-Like Growth Factor II Glycogen Receptor, Insulin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hari J
Department of Pharmacology, Stanford University School of Medicine, CA 94305-5332.
Pierce S B
Morgan D O
Sara V
Smith M C
Roth R A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-11-00
Pages
3367-71
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553792
Subset
IM
Grants
NIDDK NIH HHS · DK01393 · United States
NIDDK NIH HHS · DK34926 · United States
External Links
PubMed source PMC Full Text View journal details
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