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PMID: 19633237 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Overexpression of Mn superoxide dismutase does not increase life span in mice.

The journals of gerontology. Series A, Biological sciences and medical sciences ·Vol. 64 ·No. 11 ·2009-11-00 ·Pages 1114-25

Jang YC, Pérez VI, Song W, Lustgarten MS, Salmon AB, Mele J, Qi W, Liu Y, Liang H, Chaudhuri A, Ikeno Y, Epstein CJ, Van Remmen H, Richardson A

Abstract

Genetic manipulations of Mn superoxide dismutase (MnSOD), SOD2 expression have demonstrated that altering the level of MnSOD activity is critical for cellular function and life span in invertebrates. In mammals, Sod2 homozygous knockout mice die shortly after birth, and alterations of MnSOD levels are correlated with changes in oxidative damage and in the generation of mitochondrial reactive oxygen species. In this study, we directly tested the effects of overexpressing MnSOD in young (4-6 months) and old (26-28 months) mice on mitochondrial function, levels of oxidative damage or stress, life span, and end-of-life pathology. Our data show that an approximately twofold overexpression of MnSOD throughout life in mice resulted in decreased lipid peroxidation, increased resistance against paraquat-induced oxidative stress, and decreased age-related decline in mitochondrial ATP production. However, this change in MnSOD expression did not alter either life span or age-related pathology.

MeSH Terms
Aging/metabolism Animals Female Male Mice Mice, Inbred C57BL Mice, Transgenic Mitochondria, Muscle/physiology Muscle, Skeletal/pathology Organ Size Oxidative Stress Reactive Oxygen Species/metabolism Superoxide Dismutase/physiology
Chemicals
Reactive Oxygen Species Superoxide Dismutase superoxide dismutase 2
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Jang Youngmok C
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78245-3207, USA.
Pérez Viviana I
Song Wook
Lustgarten Michael S
Salmon Adam B
Mele James
Qi Wenbo
Liu Yuhong
Liang Hanyu
Chaudhuri Asish
Ikeno Yuji
Epstein Charles J
Van Remmen Holly
Richardson Arlan
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Article Info
Journal
The journals of gerontology. Series A, Biological sciences and medical sciences
Abbr.
J Gerontol A Biol Sci Med Sci
ISSN
1758-535X
DOI
10.1093/gerona/glp100
Published
2009-11-00
Epub
2009-00-24
Pages
1114-25
Language
English
Region
United States
NLM ID
9502837
PMCID
PMC2759571
Subset
IM
Grants
NIA NIH HHS · 1P30-AG13319 · United States
NIA NIH HHS · P01AG020591 · United States
NIA NIH HHS · P01AG19316 · United States
NIA NIH HHS · R37AG026557 · United States
External Links
PubMed source DOI: 10.1093/gerona/glp100 PMC Full Text View journal details
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