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PMID: 19633089 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Feedback control of DnaA-mediated replication initiation by replisome-associated HdaA protein in Caulobacter.

Journal of bacteriology ·Vol. 191 ·No. 18 ·2009-09-00 ·Pages 5706-16

Collier J, Shapiro L

Abstract

Chromosome replication in Caulobacter crescentus is tightly regulated to ensure that initiation occurs at the right time and only once during the cell cycle. The timing of replication initiation is controlled by both CtrA and DnaA. CtrA binds to and silences the origin. Upon the clearance of CtrA from the cell, the DnaA protein accumulates and allows loading of the replisome at the origin. Here, we identify an additional layer of replication initiation control that is mediated by the HdaA protein. In Escherichia coli, the Hda protein inactivates DnaA after replication initiation. We show that the Caulobacter HdaA homologue is necessary to restrict the initiation of DNA replication to only once per cell cycle and that it dynamically colocalizes with the replisome throughout the cell cycle. Moreover, the transcription of hdaA is directly activated by DnaA, providing a robust feedback regulatory mechanism that adjusts the levels of HdaA to inactivate DnaA.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Bacterial Proteins/genetics,metabolism Caulobacter crescentus/genetics,growth & development,metabolism Cell Cycle/genetics,physiology Culture Media DNA Replication DNA, Bacterial/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Bacterial Promoter Regions, Genetic Transcription Factors/genetics,metabolism
Chemicals
Bacterial Proteins CtrA protein, Caulobacter Culture Media DNA, Bacterial DNA-Binding Proteins DnaA protein, Bacteria Transcription Factors Adenosine Triphosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Collier Justine
Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Quartier UNIL/Sorge, Lausanne, CH 1015, Switzerland. Justine.collier@unil.ch
Shapiro Lucy
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
1098-5530
DOI
10.1128/JB.00525-09
Email
justine.collier@unil.ch
Published
2009-09-00
Epub
2009-00-24
Pages
5706-16
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC2737981
Subset
IM
Grants
NIGMS NIH HHS · R01 GM051426 · United States
NIGMS NIH HHS · R24 GM073011 · United States
NIGMS NIH HHS · R01 GM51426 · United States
NIGMS NIH HHS · R24GM073011-04 · United States
External Links
PubMed source DOI: 10.1128/JB.00525-09 PMC Full Text View journal details
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