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PMID: 11532959 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A moving DNA replication factory in Caulobacter crescentus.

The EMBO journal ·Vol. 20 ·No. 17 ·2001-09-03 ·Pages 4952-63

Jensen RB, Wang SC, Shapiro L

Abstract

The in vivo intracellular location of components of the Caulobacter replication apparatus was visualized during the cell cycle. Replisome assembly occurs at the chromosomal origin located at the stalked cell pole, coincident with the initiation of DNA replication. The replisome gradually moves to midcell as DNA replication proceeds and disassembles upon completion of DNA replication. Although the newly replicated origin regions of the chromosome are rapidly moved to opposite cell poles by an active process, the replisome appears to be an untethered replication factory that is passively displaced towards the center of the cell by the newly replicated DNA. These results are consistent with a model in which unreplicated DNA is pulled into the replication factory and newly replicated DNA is bidirectionally extruded from the complex, perhaps contributing to chromosome segregation.

MeSH Terms
Bacterial Proteins/genetics,metabolism Caulobacter crescentus/cytology,genetics,metabolism Cell Cycle Cell Division Chromosomes, Fungal/genetics DNA Helicases/metabolism DNA Replication DnaB Helicases Green Fluorescent Proteins Kinetics Luminescent Proteins/genetics Models, Genetic Recombinant Fusion Proteins/biosynthesis Replication Origin
Chemicals
Bacterial Proteins Luminescent Proteins Recombinant Fusion Proteins yellow fluorescent protein, Bacteria Green Fluorescent Proteins DNA Helicases DnaB Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jensen R B
Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.
Wang S C
Shapiro L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.17.4952
Published
2001-09-03
Pages
4952-63
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125615
Subset
IM
Grants
NIGMS NIH HHS · GM32506/5120MZ · United States
NIGMS NIH HHS · GM51426 · United States
NIGMS NIH HHS · R01 GM051426 · United States
NIGMS NIH HHS · R01 GM032506 · United States
NIGMS NIH HHS · R37 GM032506 · United States
External Links
PubMed source DOI: 10.1093/emboj/20.17.4952 PMC Full Text View journal details
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