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PMID: 10880436 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification and cell cycle control of a novel pilus system in Caulobacter crescentus.

The EMBO journal ·Vol. 19 ·No. 13 ·2000-07-03 ·Pages 3223-34

Skerker JM, Shapiro L

Abstract

Pilus assembly in CAULOBACTER: crescentus occurs during a short period of the cell cycle and pili are only present at the flagellar pole of the swarmer cell. Here we report a novel assay to visualize pili by light microscopy that led to the purification of CAULOBACTER: pili and the isolation of a cluster of seven genes, including the major pilin subunit gene pilA. This gene cluster encodes a novel group of pilus assembly proteins. We have shown that the pilA promoter is activated late in the cell cycle and that transcription of the pilin subunit plays an important role in the timing of pilus assembly. pilA transcription is regulated by the global two-component response regulator CtrA, which is essential for the expression of multiple cell cycle events, providing a direct link between assembly of the pilus organelle and bacterial cell cycle control.

MeSH Terms
Amino Acid Sequence Bacteriophages/genetics Caulobacter crescentus/cytology,genetics Cell Cycle DNA, Bacterial Fimbriae, Bacterial/chemistry,genetics Fluorescence Genes, Bacterial Molecular Sequence Data Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Skerker J M
Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, CA 94305, USA.
Shapiro L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.13.3223
Published
2000-07-03
Pages
3223-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC313932
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032506 · United States
NIGMS NIH HHS · R37 GM032506 · United States
NIGMS NIH HHS · GM32506/5120MZ · United States
External Links
PubMed source DOI: 10.1093/emboj/19.13.3223 PMC Full Text View journal details
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