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PMID: 19627584 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

YA is needed for proper nuclear organization to transition between meiosis and mitosis in Drosophila.

BMC developmental biology ·Vol. 9 ·2009-07-23 ·Pages 43

Sackton KL, Lopez JM, Berman CL, Wolfner MF

Abstract

The Drosophila YA protein is required to initiate the embryonic cleavage divisions. After egg activation, YA enters nuclei and interacts with chromatin and the nuclear lamina. This study was designed to define more precisely the events prior to the first cleavage division that are dependent upon YA. We find that meiosis is completed normally in the absence of YA function. The first defects in embryos and eggs from mutant mothers first appear just after the completion of meiosis, and are seen as abnormal associations among the resultant haploid nuclei. These defects are associated with asynchronies in the cell cycle-dependent chromatin condensation state of the haploid nuclei. However, we find evidence of DNA replication in the absence of YA function. Our data suggest YA function is needed at a control point, following meiosis II and the initiation of the first postmeiotic S phase, which is sensitive to the chromatin condensation state of the haploid meiotic products.

MeSH Terms
Animals Chromosomal Proteins, Non-Histone/genetics,metabolism,physiology DNA-Binding Proteins/genetics,metabolism,physiology Drosophila/cytology,embryology,metabolism Drosophila Proteins/genetics,metabolism,physiology Fluorescent Antibody Technique Meiosis/genetics,physiology Microscopy Mitosis/genetics,physiology
Chemicals
Chromosomal Proteins, Non-Histone DNA-Binding Proteins Drosophila Proteins fs(1)Ya protein, Drosophila
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sackton Katharine L
Dept of Molecular Biology and Genetics, Cornell University, Ithaca NY 14853, USA. klb56@cornell.edu
Lopez Jacqueline M
Berman Cindy L
Wolfner Mariana F
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Article Info
Journal
BMC developmental biology
Abbr.
BMC Dev Biol
ISSN
1471-213X
DOI
10.1186/1471-213X-9-43
Email
klb56@cornell.edu
Published
2009-07-23
Epub
2009-00-23
Pages
43
Language
English
Region
England
NLM ID
100966973
PMCID
PMC2724486
Subset
IM
Grants
NIGMS NIH HHS · T32 GM007617 · United States
NIGMS NIH HHS · GM44659 · United States
NIGMS NIH HHS · GM07617 · United States
NIGMS NIH HHS · GM07273 · United States
NIGMS NIH HHS · T32 GM007273 · United States
NIGMS NIH HHS · R01 GM044659 · United States
External Links
PubMed source DOI: 10.1186/1471-213X-9-43 PMC Full Text View journal details
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