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PMID: 19623612 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Transcription factor GATA-3 is essential for lens development.

Developmental dynamics : an official publication of the American Association of Anatomists ·Vol. 238 ·No. 9 ·2009-09-00 ·Pages 2280-91

Maeda A, Moriguchi T, Hamada M, Kusakabe M, Fujioka Y, Nakano T, Yoh K, Lim KC, Engel JD, Takahashi S

Abstract

During vertebrate lens development, the anterior, ectoderm-derived lens vesicle cells differentiate into a monolayer of epithelial cells that retain proliferative potential. Subsequently, they exit the cell cycle and give rise to posterior lens fiber cells that form the lens body. In the present study, we demonstrate that the transcription factor GATA-3 is expressed in the posterior lens fiber cells during embryogenesis, and that GATA-3 deficiency impairs lens development. Interestingly, expression of E-cadherin, a premature lens vesicle marker, is abnormally prolonged in the posterior region of Gata3 homozygous mutant lenses. Furthermore, expression of gamma-crystallin, a differentiation marker for fiber cells, is reduced. This suppressed differentiation is accompanied by an abnormal cellular proliferation, as well as with diminished levels of the cell-cycle inhibitors Cdkn1b/p27 and Cdkn1c/p57 and increased Ccnd2/cyclin D2 abundance. Thus, these observations suggest that GATA-3 is essential for lens cells differentiation and proper cell cycle control.

MeSH Terms
Animals Apoptosis Cadherins/genetics,physiology Cell Cycle/genetics,physiology Cell Differentiation/genetics,physiology Cyclin D2 Cyclin-Dependent Kinase Inhibitor p27/genetics,physiology Cyclins/genetics,physiology Flow Cytometry Fluorescent Antibody Technique GATA3 Transcription Factor/genetics,metabolism Gene Expression Regulation, Developmental In Situ Nick-End Labeling Lens, Crystalline/cytology,embryology Mice Mice, Knockout Reverse Transcriptase Polymerase Chain Reaction gamma-Crystallins/genetics,physiology
Chemicals
Cadherins Ccnd2 protein, mouse Cdkn1b protein, mouse Cyclin D2 Cyclins GATA3 Transcription Factor Gata3 protein, mouse gamma-Crystallins Cyclin-Dependent Kinase Inhibitor p27
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Maeda Atsuko
Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan.
Moriguchi Takashi
Hamada Michito
Kusakabe Manabu
Fujioka Yuki
Nakano Takako
Yoh Keigyou
Lim Kim-Chew
Engel James Douglas
Takahashi Satoru
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Article Info
Journal
Developmental dynamics : an official publication of the American Association of Anatomists
Abbr.
Dev Dyn
ISSN
1097-0177
DOI
10.1002/dvdy.22035
Published
2009-09-00
Pages
2280-91
Language
English
Region
United States
NLM ID
9201927
PMCID
PMC3408506
Subset
IM
Grants
NIGMS NIH HHS · R01 GM028896 · United States
NIGMS NIH HHS · GM28896 · United States
External Links
PubMed source DOI: 10.1002/dvdy.22035 PMC Full Text View journal details
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