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PMID: 17306631 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Pax6 is misexpressed in Sox1 null lens fiber cells.

Gene expression patterns : GEP ·Vol. 7 ·No. 5 ·2007-04-00 ·Pages 606-13

Donner AL, Ko F, Episkopou V, Maas RL

Abstract

Sox1 null lens fiber cells fail to elongate and have disrupted expression of gamma-crystallin. We have evaluated the expression of Sox1 and Pax6 proteins during critical stages of lens morphogenesis, with particular focus on fiber cell differentiation. While Pax6 and Sox1 are co-expressed during early stages of fiber cell differentiation, Sox1 up-regulation coincides temporally with the down-regulation of Pax6, and these proteins therefore display a striking inverse expression pattern in the lens fiber cell compartment. Furthermore, Pax6 is inappropriately expressed in the fiber cells of Sox1 null mice and the Pax6 target, alpha5 integrin, is simultaneously misexpressed. Finally, we demonstrate a genetic interaction between Sox1 and Pax6, as Sox1 heterozygosity partially rescues the diameter of Pax6(Sey) lenses by increasing the number of cells in the fiber cell compartment.

MeSH Terms
Animals Crosses, Genetic DNA-Binding Proteins/physiology Embryo, Mammalian/cytology,metabolism Eye Proteins/physiology Female Fluorescent Antibody Technique Heterozygote High Mobility Group Proteins/physiology Homeodomain Proteins/physiology Lens, Crystalline/cytology,metabolism Male Mice PAX6 Transcription Factor Paired Box Transcription Factors/physiology Repressor Proteins/physiology SOXB1 Transcription Factors gamma-Crystallins/genetics
Chemicals
DNA-Binding Proteins Eye Proteins High Mobility Group Proteins Homeodomain Proteins PAX6 Transcription Factor Paired Box Transcription Factors Pax6 protein, mouse Repressor Proteins SOXB1 Transcription Factors Sox1 protein, mouse gamma-Crystallins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Donner Amy L
Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, NRB 458, 77 Louis Pasteur Avenue, Boston, MA 02115, USA. adonner@rics.bwh.harvard.edu
Ko Fang
Episkopou Vasso
Maas Richard L
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Article Info
Journal
Gene expression patterns : GEP
Abbr.
Gene Expr Patterns
ISSN
1567-133X
DOI
10.1016/j.modgep.2007.01.001
Email
adonner@rics.bwh.harvard.edu
Published
2007-04-00
Epub
2007-00-11
Pages
606-13
Language
English
Region
Netherlands
NLM ID
101167473
PMCID
PMC2246053
Subset
IM
Grants
NIDCR NIH HHS · F32-DE05735 · United States
NEI NIH HHS · T32-EY07145 · United States
NEI NIH HHS · R01 EY010123-07 · United States
NEI NIH HHS · R01 EY010123-06 · United States
NEI NIH HHS · R01 EY010123-10 · United States
NIDCR NIH HHS · F32 DE005735 · United States
Medical Research Council · MC_U120074332 · United Kingdom
NEI NIH HHS · R01 EY010123 · United States
NEI NIH HHS · T32 EY007145 · United States
External Links
PubMed source DOI: 10.1016/j.modgep.2007.01.001 PMC Full Text View journal details
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