Home LiteratureArticle Details
PMID: 19605552 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Molecular and chromosomal evidence for allopolyploidy in soybean.

Plant physiology ·Vol. 151 ·No. 3 ·2009-11-00 ·Pages 1167-74

Gill N, Findley S, Walling JG, Hans C, Ma J, Doyle J, Stacey G, Jackson SA

Abstract

Recent studies have documented that the soybean (Glycine max) genome has undergone two rounds of large-scale genome and/or segmental duplication. To shed light on the timing and nature of these duplication events, we characterized and analyzed two subfamilies of high-copy centromeric satellite repeats, CentGm-1 and CentGm-2, using a combination of computational and molecular cytogenetic approaches. These two subfamilies of satellite repeats mark distinct subsets of soybean centromeres and, in at least one case, a pair of homologs, suggesting their origins from an allopolyploid event. The satellite monomers of each subfamily are arranged in large tandem arrays, and intermingled monomers of the two subfamilies were not detected by fluorescence in situ hybridization on extended DNA fibers nor at the sequence level. This indicates that there has been little recombination and homogenization of satellite DNA between these two sets of centromeres. These satellite repeats are also present in Glycine soja, the proposed wild progenitor of soybean, but could not be detected in any other relatives of soybean examined in this study, suggesting the rapid divergence of the centromeric satellite DNA within the Glycine genus. Together, these observations provide direct evidence, at molecular and chromosomal levels, in support of the hypothesis that the soybean genome has experienced a recent allopolyploidization event.

MeSH Terms
Centromere/genetics Chromosomes, Plant Computational Biology DNA, Plant/genetics DNA, Satellite/genetics Evolution, Molecular Genome, Plant In Situ Hybridization, Fluorescence Molecular Sequence Data Phylogeny Polyploidy Soybeans/genetics
Chemicals
DNA, Plant DNA, Satellite
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gill Navdeep
Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, USA.
Findley Seth
Walling Jason G
Hans Christian
Ma Jianxin
Doyle Jeff
Stacey Gary
Jackson Scott A
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.109.137935
Published
2009-11-00
Epub
2009-00-15
Pages
1167-74
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2773056
Subset
IM
Databases
GENBANK
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PubMed source DOI: 10.1104/pp.109.137935 PMC Full Text View journal details
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