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PMID: 15879505 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Pericentromeric regions of soybean (Glycine max L. Merr.) chromosomes consist of retroelements and tandemly repeated DNA and are structurally and evolutionarily labile.

Genetics ·Vol. 170 ·No. 3 ·2005-07-00 ·Pages 1221-30

Lin JY, Jacobus BH, SanMiguel P, Walling JG, Yuan Y, Shoemaker RC, Young ND, Jackson SA

Abstract

Little is known about the physical makeup of heterochromatin in the soybean (Glycine max L. Merr.) genome. Using DNA sequencing and molecular cytogenetics, an initial analysis of the repetitive fraction of the soybean genome is presented. BAC 076J21, derived from linkage group L, has sequences conserved in the pericentromeric heterochromatin of all 20 chromosomes. FISH analysis of this BAC and three subclones on pachytene chromosomes revealed relatively strict partitioning of the heterochromatic and euchromatic regions. Sequence analysis showed that this BAC consists primarily of repetitive sequences such as a 102-bp tandem repeat with sequence identity to a previously characterized approximately 120-bp repeat (STR120). Fragments of Calypso-like retroelements, a recently inserted SIRE1 element, and a SIRE1 solo LTR were present within this BAC. Some of these sequences are methylated and are not conserved outside of G. max and G. soja, a close relative of soybean, except for STR102, which hybridized to a restriction fragment from G. latifolia. These data present a picture of the repetitive fraction of the soybean genome that is highly concentrated in the pericentromeric regions, consisting of rapidly evolving tandem repeats with interspersed retroelements.

MeSH Terms
Base Sequence Blotting, Southern Chromosomes, Artificial, Bacterial Chromosomes, Plant/genetics Evolution, Molecular Heterochromatin/genetics In Situ Hybridization, Fluorescence Molecular Sequence Data Retroelements/genetics Sequence Analysis, DNA Soybeans/genetics Tandem Repeat Sequences/genetics
Chemicals
Heterochromatin Retroelements
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lin Jer-Young
Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, USA.
Jacobus Barbara Hass
SanMiguel Phillip
Walling Jason G
Yuan Yinan
Shoemaker Randy C
Young Nevin D
Jackson Scott A
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.105.041616
Published
2005-07-00
Epub
2005-00-06
Pages
1221-30
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1451161
Subset
IM
Databases
GENBANK
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PubMed source DOI: 10.1534/genetics.105.041616 PMC Full Text View journal details
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