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PMID: 19605494 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis.

Development (Cambridge, England) ·Vol. 136 ·No. 16 ·2009-08-00 ·Pages 2815-23

Driskell RR, Giangreco A, Jensen KB, Mulder KW, Watt FM

Abstract

The dermal papilla comprises the specialised mesenchymal cells at the base of the hair follicle. Communication between dermal papilla cells and the overlying epithelium is essential for differentiation of the hair follicle lineages. We report that Sox2 is expressed in all dermal papillae at E16.5, but from E18.5 onwards expression is confined to a subset of dermal papillae. In postnatal skin, Sox2 is only expressed in the dermal papillae of guard/awl/auchene follicles, whereas CD133 is expressed both in guard/awl/auchene and in zigzag dermal papillae. Using transgenic mice that express GFP under the control of the Sox2 promoter, we isolated Sox2(+) (GFP(+)) CD133(+) cells and compared them with Sox2(-) (GFP(-)) CD133(+) dermal papilla cells. In addition to the 'core' dermal papilla gene signature, each subpopulation expressed distinct sets of genes. GFP(+) CD133(+) cells had upregulated Wnt, FGF and BMP pathways and expressed neural crest markers. In GFP(-) CD133(+) cells, the hedgehog, IGF, Notch and integrin pathways were prominent. In skin reconstitution assays, hair follicles failed to form when dermis was depleted of both GFP(+) CD133(+) and GFP(-) CD133(+) cells. In the absence of GFP(+) CD133(+) cells, awl/auchene hairs failed to form and only zigzag hairs were found. We have thus demonstrated a previously unrecognised heterogeneity in dermal papilla cells and shown that Sox2-positive cells specify particular hair follicle types.

MeSH Terms
AC133 Antigen Animals Antigens, CD/genetics,metabolism Dermis/cytology,embryology Embryo, Mammalian/anatomy & histology,physiology Epidermal Cells Epidermis/embryology,metabolism Gene Expression Profiling Gene Regulatory Networks Glycoproteins/genetics,metabolism Hair Follicle/cytology,embryology,metabolism Mice Mice, Inbred BALB C Mice, Nude Mice, Transgenic Oligonucleotide Array Sequence Analysis Peptides/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism SOXB1 Transcription Factors/genetics,metabolism Signal Transduction/physiology
Chemicals
AC133 Antigen Antigens, CD Glycoproteins Peptides Prom1 protein, mouse Recombinant Fusion Proteins SOXB1 Transcription Factors Sox2 protein, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Driskell Ryan R
Wellcome Trust Centre for Stem Cell Research, Cambridge, UK.
Giangreco Adam
Jensen Kim B
Mulder Klaas W
Watt Fiona M
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Article Info
Journal
Development (Cambridge, England)
Abbr.
Development
ISSN
1477-9129
DOI
10.1242/dev.038620
Published
2009-08-00
Epub
2009-00-15
Pages
2815-23
Language
English
Region
England
NLM ID
8701744
PMCID
PMC2730408
Subset
IM
Grants
Wellcome Trust · United Kingdom
Medical Research Council · G0300723 · United Kingdom
Medical Research Council · G0800784 · United Kingdom
Medical Research Council · G1100073 · United Kingdom
Cancer Research UK · United Kingdom
External Links
PubMed source DOI: 10.1242/dev.038620 PMC Full Text View journal details
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