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PMID: 19582558 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Elucidating anomalous protein diffusion in living cells with fluorescence correlation spectroscopy-facts and pitfalls.

Journal of fluorescence ·Vol. 20 ·No. 1 ·2010-01-00 ·Pages 19-26

Malchus N, Weiss M

Abstract

Anomalous protein diffusion has been frequently observed in intracellular fluids and on membranes of living cells. Indeed, a large variety of specimen, from bacteriae to mammalian cells, and several non-invasive measurement techniques, e.g. fluorescence correlation spectroscopy, have revealed that the mean square displacement (MSD) of proteins in vivo is often characterized by an anomalous power-law increase mean value of tau(t)(2) mean value of ~ t(alpha) with 0.5 < alpha </= 0.8. Here, we review these results with a particular focus on fluorescence correlation spectroscopy, and we report on possible causes of variations of the anomaly degree alpha. Moreover, we highlight generic consequences of anomalous diffusion that are likely to play an important role in the cellular context.

MeSH Terms
Cell Membrane/metabolism Cell Survival Diffusion HeLa Cells Humans Intracellular Fluid/metabolism Proteins/metabolism Spectrometry, Fluorescence/methods
Chemicals
Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Malchus Nina
Cellular Biophysics Group, German Cancer Research Center, c/o BIOQUANT, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany.
Weiss Matthias
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Article Info
Journal
Journal of fluorescence
Abbr.
J Fluoresc
ISSN
1573-4994
DOI
10.1007/s10895-009-0517-4
Published
2010-01-00
Epub
2009-00-07
Pages
19-26
Language
English
Region
Netherlands
NLM ID
9201341
Subset
IM
External Links
PubMed source DOI: 10.1007/s10895-009-0517-4 View journal details
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