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PMID: 19544429 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Epidermal growth factor induces the progeny of subventricular zone type B cells to migrate and differentiate into oligodendrocytes.

Stem cells (Dayton, Ohio) ·Vol. 27 ·No. 8 ·2009-08-00 ·Pages 2032-43

Gonzalez-Perez O, Romero-Rodriguez R, Soriano-Navarro M, Garcia-Verdugo JM, Alvarez-Buylla A

Abstract

New neurons and oligodendrocytes are continuously produced in the subventricular zone (SVZ) of adult mammalian brains. Under normal conditions, the SVZ primary precursors (type B1 cells) generate type C cells, most of which differentiate into neurons, with a small subpopulation giving rise to oligodendrocytes. Epidermal growth factor (EGF) signaling induces dramatic proliferation and migration of SVZ progenitors, a process that could have therapeutic applications. However, the fate of cells derived from adult neural stem cells after EGF stimulation remains unknown. Here, we specifically labeled SVZ B1 cells and followed their progeny after a 7-day intraventricular infusion of EGF. Cells derived from SVZ B1 cells invaded the parenchyma around the SVZ into the striatum, septum, corpus callosum, and fimbria-fornix. Most of these B1-derived cells gave rise to cells in the oligodendrocyte lineage, including local NG2+ progenitors, and pre-myelinating and myelinating oligodendrocytes. SVZ B1 cells also gave rise to a population of highly-branched S100beta+/glial fibrillary acidic protein (GFAP)+ cells in the striatum and septum, but no neuronal differentiation was observed. Interestingly, when demyelination was induced in the corpus callosum by a local injection of lysolecithin, an increased number of cells derived from SVZ B1 cells and stimulated to migrate and proliferate by EGF infusion differentiated into oligodendrocytes at the lesion site. This work indicates that EGF infusion can greatly expand the number of progenitors derived from the SVZ primary progenitors which migrate and differentiate into oligodendroglial cells. This expanded population could be used for the repair of white matter lesions.

MeSH Terms
Adult Animals Cell Differentiation/drug effects,physiology Cell Lineage Cell Movement/drug effects,physiology Cell Proliferation Cerebral Ventricles/cytology,metabolism Demyelinating Diseases Epidermal Growth Factor/metabolism,pharmacology Humans Mice Myelin Sheath/metabolism Neuroendocrine Cells/cytology Neurons/cytology,drug effects,metabolism Oligodendroglia/cytology,drug effects,metabolism
Chemicals
Epidermal Growth Factor
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gonzalez-Perez Oscar
Department of Neurological Surgery, Brain Tumor Research Center, Institute for Regeneration Medicine, University of California, San Francisco, California, USA.
Romero-Rodriguez Ricardo
Soriano-Navarro Mario
Garcia-Verdugo Jose Manuel
Alvarez-Buylla Arturo
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Article Info
Journal
Stem cells (Dayton, Ohio)
Abbr.
Stem Cells
ISSN
1549-4918
DOI
10.1002/stem.119
Published
2009-08-00
Pages
2032-43
Language
English
Region
United States
NLM ID
9304532
PMCID
PMC3346259
Subset
IM
Grants
NICHD NIH HHS · HD 32116 · United States
NICHD NIH HHS · R37 HD032116-15 · United States
NICHD NIH HHS · R37 HD032116 · United States
NICHD NIH HHS · R01 HD032116 · United States
NICHD NIH HHS · R37 HD032116-16 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1002/stem.119 PMC Full Text View journal details
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