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PMID: 16319309 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Overexpression of the epidermal growth factor receptor confers migratory properties to nonmigratory postnatal neural progenitors.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 25 ·No. 48 ·2005-11-30 ·Pages 11092-106

Aguirre A, Rizvi TA, Ratner N, Gallo V

Abstract

Approaches to successful cell transplantation therapies for the injured brain involve selecting the appropriate neural progenitor type and optimizing the efficiency of the cell engraftment. Here we show that epidermal growth factor receptor (EGFR) expression enhances postnatal neural progenitor migration in vitro and in vivo. Migratory NG2-expressing (NG2+) progenitor cells of the postnatal subventricular zone (SVZ) express higher EGFR levels than nonmigratory, cortical NG2+ cells. The higher endogenous EGFR expression in SVZ NG2+ cells is causally related with their migratory potential in vitro as well as in vivo after cell engraftment. EGFR overexpression in cortical NG2+ cells by transient transfection converted these cells to a migratory phenotype in vitro and in vivo. Finally, cortical NG2+ cells purified from a transgenic mouse in which the EGFR is overexpressed under the CNP promoter exhibited enhanced migratory capability. These findings reveal a new role for EGFR in the postnatal brain and open new avenues to optimize cell engraftment for brain repair.

MeSH Terms
Animals Animals, Newborn/physiology Antigens/genetics,metabolism,physiology Brain/cytology Cell Movement/physiology Cerebral Cortex/cytology Cerebral Ventricles Epidermal Growth Factor/metabolism ErbB Receptors/genetics,physiology Extracellular Matrix Proteins/physiology Humans Mice Mice, Transgenic Natriuretic Peptide, C-Type/metabolism Neurons/metabolism,physiology,transplantation Phenotype Proteoglycans/genetics,metabolism,physiology Stem Cell Transplantation Stem Cells/metabolism,physiology Transfection
Chemicals
Antigens Extracellular Matrix Proteins Proteoglycans chondroitin sulfate proteoglycan 4 Natriuretic Peptide, C-Type Epidermal Growth Factor ErbB Receptors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Aguirre Adan
Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center, Washington, DC 20010, USA.
Rizvi Tilat A
Ratner Nancy
Gallo Vittorio
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.2981-05.2005
Published
2005-11-30
Pages
11092-106
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6725641
Subset
IM
Grants
NINDS NIH HHS · R01 NS045702 · United States
NICHD NIH HHS · P30 HD040677 · United States
NINDS NIH HHS · R01NS028840 · United States
NINDS NIH HHS · R01NS045702 · United States
NICHD NIH HHS · P30HD40677 · United States
NINDS NIH HHS · R01 NS028840 · United States
External Links
PubMed source DOI: 10.1523/JNEUROSCI.2981-05.2005 PMC Full Text View journal details
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