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PMID: 195089 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell-free synthesis of herpes simplex virus proteins.

Journal of virology ·Vol. 22 ·No. 3 ·1977-06-00 ·Pages 750-7

Cremer KJ, Summers WC, Gesteland RF

Abstract

Polyribosomes isolated from herpes simplex virus type I (HSV-1)-infected cells have been used to program a eucaryotic cell-free translation system. At least 10 HSV-specific polypeptides, with apparent molecular weights of 25,000 to 160,000, are synthesized by wild-type HSV-infected polyribosomes. Polyribosomes prepared from thymidine kinase-negative mutants of HSV direct the synthesis of three putative nonsense termination polypeptides. HSV-specific polypeptides synthesized in vitro are precipitated with antiserum to HSV-infected cell proteins.

MeSH Terms
Animals Cell Line Cell-Free System Haplorhini Magnesium/pharmacology Molecular Weight Peptide Biosynthesis Polyribosomes/metabolism Putrescine/metabolism Simplexvirus/immunology,metabolism Thymidine Kinase/metabolism Viral Proteins/biosynthesis,immunology
Chemicals
Viral Proteins Thymidine Kinase Magnesium Putrescine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cremer K J
Summers W C
Gesteland R F
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22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.22.3.750-757.1977
Published
1977-06-00
Pages
750-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515775
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.22.3.750-757.1977 PMC Full Text View journal details
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