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PMID: 19483686 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Brain extracellular matrix affects AMPA receptor lateral mobility and short-term synaptic plasticity.

Nature neuroscience ·Vol. 12 ·No. 7 ·2009-07-00 ·Pages 897-904

Frischknecht R, Heine M, Perrais D, Seidenbecher CI, Choquet D, Gundelfinger ED

Abstract

Many synapses in the mature CNS are wrapped by a dense extracellular matrix (ECM). Using single-particle tracking and fluorescence recovery after photobleaching, we found that this net-like ECM formed surface compartments on rat primary neurons that acted as lateral diffusion barriers for AMPA-type glutamate receptors. Enzymatic removal of the ECM increased extrasynaptic receptor diffusion and the exchange of synaptic AMPA receptors. Whole-cell patch-clamp recording revealed an increased paired-pulse ratio as a functional consequence of ECM removal. These results suggest that the surface compartments formed by the ECM hinder lateral diffusion of AMPA receptors and may therefore modulate short-term synaptic plasticity.

MeSH Terms
Animals Brain/physiology Cells, Cultured Dendrites/physiology Extracellular Matrix/physiology Fluorescence Recovery After Photobleaching Hyaluronoglucosaminidase/metabolism In Vitro Techniques Mice Neuronal Plasticity/physiology Neurons/physiology Patch-Clamp Techniques Rats Rats, Sprague-Dawley Receptors, AMPA/metabolism Receptors, N-Methyl-D-Aspartate/metabolism Synapses/physiology Synaptic Transmission/physiology
Chemicals
NR1 NMDA receptor NR2A NMDA receptor Receptors, AMPA Receptors, N-Methyl-D-Aspartate Hyaluronoglucosaminidase glutamate receptor ionotropic, AMPA 2 glutamate receptor ionotropic, AMPA 1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Frischknecht Renato
Leibniz Institute for Neurobiology, Magdeburg, Germany.
Heine Martin
Perrais David
Seidenbecher Constanze I
Choquet Daniel
Gundelfinger Eckart D
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Article Info
Journal
Nature neuroscience
Abbr.
Nat Neurosci
ISSN
1546-1726
DOI
10.1038/nn.2338
Published
2009-07-00
Epub
2009-00-31
Pages
897-904
Language
English
Region
United States
NLM ID
9809671
Subset
IM
External Links
PubMed source DOI: 10.1038/nn.2338 View journal details
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