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PMID: 18403705 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Surface mobility of postsynaptic AMPARs tunes synaptic transmission.

Science (New York, N.Y.) ·Vol. 320 ·No. 5873 ·2008-04-11 ·Pages 201-5

Heine M, Groc L, Frischknecht R, Béïque JC, Lounis B, Rumbaugh G, Huganir RL, Cognet L, Choquet D

Abstract

AMPA glutamate receptors (AMPARs) mediate fast excitatory synaptic transmission. Upon fast consecutive synaptic stimulation, transmission can be depressed. Recuperation from fast synaptic depression has been attributed solely to recovery of transmitter release and/or AMPAR desensitization. We show that AMPAR lateral diffusion, observed in both intact hippocampi and cultured neurons, allows fast exchange of desensitized receptors with naïve functional ones within or near the postsynaptic density. Recovery from depression in the tens of millisecond time range can be explained in part by this fast receptor exchange. Preventing AMPAR surface movements through cross-linking, endogenous clustering, or calcium rise all slow recovery from depression. Physiological regulation of postsynaptic receptor mobility affects the fidelity of synaptic transmission by shaping the frequency dependence of synaptic responses.

MeSH Terms
Action Potentials Animals Calcium/metabolism Cells, Cultured Diffusion Excitatory Amino Acid Antagonists/pharmacology Excitatory Postsynaptic Potentials Fluorescence Recovery After Photobleaching Glutamic Acid/metabolism Hippocampus/cytology,physiology Kynurenic Acid/pharmacology Neuronal Plasticity Neurons/physiology Patch-Clamp Techniques Rats Rats, Sprague-Dawley Receptors, AMPA/metabolism Recombinant Fusion Proteins/metabolism Synapses/drug effects,physiology Synaptic Transmission/drug effects Transfection
Chemicals
Excitatory Amino Acid Antagonists Receptors, AMPA Recombinant Fusion Proteins Glutamic Acid Kynurenic Acid glutamate receptor ionotropic, AMPA 2 Calcium glutamate receptor ionotropic, AMPA 1
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Heine Martin
CNRS, UMR 5091, Université Bordeaux, Bordeaux, France.
Groc Laurent
Frischknecht Renato
Béïque Jean-Claude
Lounis Brahim
Rumbaugh Gavin
Huganir Richard L
Cognet Laurent
Choquet Daniel
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1152089
Published
2008-04-11
Pages
201-5
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC2715948
Subset
IM
Grants
Howard Hughes Medical Institute · United States
Corrections
CommentIn
PMID: 18403696
External Links
PubMed source DOI: 10.1126/science.1152089 PMC Full Text View journal details
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