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PMID: 7711235 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The mechanism of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor desensitization after removal of glutamate.

Biophysical journal ·Vol. 68 ·No. 1 ·1995-01-00 ·Pages 137-46

Raman IM, Trussell LO

Abstract

We have examined responses of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate) receptors in the chick nucleus magnocellularis to pairs of pulses of glutamate and determined the extent of desensitization and the rate of recovery. Receptors recovered from desensitization with a time constant of 16 ms, regardless of the concentration or duration of the conditioning pulse. Even with very brief conditioning pulses, evoking submaximal currents, desensitization occurred at a consistent rate after the removal of free ligand. A quantitative kinetic model based on these data shows that receptors must desensitize from a closed state. The results provide evidence that very brief exposure to glutamate, on the time scale of uniquantal synaptic transmission, will result in a significant reduction in sensitivity of postsynaptic receptors.

MeSH Terms
Animals Biophysical Phenomena Biophysics Chick Embryo Cochlear Nucleus/cytology,drug effects,metabolism Glutamic Acid/pharmacology In Vitro Techniques Kainic Acid/pharmacology Kinetics Models, Neurological Neurons/drug effects,metabolism Receptors, AMPA/drug effects,metabolism
Chemicals
Receptors, AMPA Glutamic Acid Kainic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Raman I M
Neuroscience Training Program, University of Wisconsin Medical School, Madison 53706.
Trussell L O
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(95)80168-2
Published
1995-01-00
Pages
137-46
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1281670
Subset
IM
Grants
NINDS NIH HHS · NS28901 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(95)80168-2 PMC Full Text View journal details
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