Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.

Nucleic acids research ·Vol. 4 ·No. 4 ·1977-04-00 ·Pages 1001-14

Contreras R, Volckaert G, Thys F, Van de Voorde A, Fiers W

Abstract

The nucleotide sequence of the SV40 genome region between the Hind K fragment and the Eco RI cleavage site has been determined by a combination of three different approaches : analysis of RNA products obtained by transcription with Escherichia coli DNA dependent RNA polymerase, partial degradations with snake venom exonuclease and base-specific chemical degradation of 5'-terminal labeled restriction fragments. This nucleotide sequence shows only one open reading frame and allows the deduction of a small segment of the amino acid sequence of VP1, the major structural protein.

MeSH Terms

Alkaline Phosphatase Base Sequence Chemical Phenomena Chemistry DNA Restriction Enzymes DNA, Viral DNA-Directed RNA Polymerases/metabolism Deoxyribonucleotides/analysis,metabolism Escherichia coli/enzymology Exonucleases Genotype Polynucleotide 5'-Hydroxyl-Kinase RNA/biosynthesis Ribonucleases Simian virus 40 Snake Venoms Transcription, Genetic

Chemicals

DNA, Viral Deoxyribonucleotides Snake Venoms RNA Polynucleotide 5'-Hydroxyl-Kinase DNA-Directed RNA Polymerases Exonucleases Ribonucleases DNA Restriction Enzymes Alkaline Phosphatase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Contreras R

Volckaert G

Thys F

Van de Voorde A

Fiers W

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.4.1001

Published

1977-04-00

Pages

1001-14

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342500

Subset

Databases

GENBANK

J02400, J02402, J02403, J02406, J02407, J02408, J02409, J02410, V01380

External Links

PubMed source DOI: 10.1093/nar/4.4.1001 PMC Full Text View journal details

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Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.

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