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PMID: 19395410 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The cytokinin type-B response regulator PtRR13 is a negative regulator of adventitious root development in Populus.

Plant physiology ·Vol. 150 ·No. 2 ·2009-06-00 ·Pages 759-71

Ramírez-Carvajal GA, Morse AM, Dervinis C, Davis JM

Abstract

Adventitious root formation at the base of plant cuttings is an innate de novo organogenesis process that allows massive vegetative propagation of many economically and ecologically important species. The early molecular events following shoot excision are not well understood. Using whole-genome microarrays, we detected significant transcriptome remodeling during 48 h following shoot removal in Populus tremula x Populus alba softwood cuttings in the absence of exogenous auxin, with 27% and 36% of the gene models showing differential abundance between 0 and 6 h and between 6 and 24 h, respectively. During these two time intervals, gene networks involved in protein turnover, protein phosphorylation, molecular transport, and translation were among the most significantly regulated. Transgenic lines expressing a constitutively active form of the Populus type-B cytokinin response regulator PtRR13 (DeltaDDKPtRR13) have a delayed rooting phenotype and cause misregulation of CONTINUOUS VASCULAR RING1, a negative regulator of vascularization; PLEIOTROPIC DRUG RESISTANCE TRANSPORTER9, an auxin efflux transporter; and two APETALA2/ETHYLENE RESPONSE FACTOR genes with sequence similarity to TINY. Inappropriate cytokinin action via DeltaDDKPtRR13 expression appeared to disrupt adventitious root development 24 h after shoot excision, when root founder cells are hypothesized to be sensitive to the negative effects of cytokinin. Our results are consistent with PtRR13 acting downstream of cytokinin to repress adventitious root formation in intact plants, and that reduced cytokinin signaling after shoot excision enables coordinated expression of ethylene, auxin, and vascularization pathways leading to adventitious root development.

MeSH Terms
Cytokinins/metabolism Ethylenes/metabolism Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Indoleacetic Acids/metabolism Models, Biological Multigene Family Phenotype Plant Proteins/genetics,metabolism Plant Roots/genetics,growth & development Plant Shoots/genetics Plants, Genetically Modified Populus/genetics,growth & development Protein Structure, Tertiary RNA Interference
Chemicals
Cytokinins Ethylenes Indoleacetic Acids Plant Proteins ethylene
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ramírez-Carvajal Gustavo A
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA.
Morse Alison M
Dervinis Christopher
Davis John M
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.109.137505
Published
2009-06-00
Epub
2009-00-24
Pages
759-71
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2689991
Subset
IM
Corrections
CommentIn
PMID: 20037469
External Links
PubMed source DOI: 10.1104/pp.109.137505 PMC Full Text View journal details
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