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PMID: 18065686 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cytokinins act directly on lateral root founder cells to inhibit root initiation.

The Plant cell ·Vol. 19 ·No. 12 ·2007-12-00 ·Pages 3889-900

Laplaze L, Benkova E, Casimiro I, Maes L, Vanneste S, Swarup R, Weijers D, Calvo V, Parizot B, Herrera-Rodriguez MB, Offringa R, Graham N, Doumas P, Friml J, Bogusz D, Beeckman T, Bennett M

Abstract

In Arabidopsis thaliana, lateral roots are formed from root pericycle cells adjacent to the xylem poles. Lateral root development is regulated antagonistically by the plant hormones auxin and cytokinin. While a great deal is known about how auxin promotes lateral root development, the mechanism of cytokinin repression is still unclear. Elevating cytokinin levels was observed to disrupt lateral root initiation and the regular pattern of divisions that characterizes lateral root development in Arabidopsis. To identify the stage of lateral root development that is sensitive to cytokinins, we targeted the expression of the Agrobacterium tumefaciens cytokinin biosynthesis enzyme isopentenyltransferase to either xylem-pole pericycle cells or young lateral root primordia using GAL4-GFP enhancer trap lines. Transactivation experiments revealed that xylem-pole pericycle cells are sensitive to cytokinins, whereas young lateral root primordia are not. This effect is physiologically significant because transactivation of the Arabidopsis cytokinin degrading enzyme cytokinin oxidase 1 in lateral root founder cells results in increased lateral root formation. We observed that cytokinins perturb the expression of PIN genes in lateral root founder cells and prevent the formation of an auxin gradient that is required to pattern lateral root primordia.

MeSH Terms
Agrobacterium tumefaciens/genetics Alkyl and Aryl Transferases/genetics,metabolism Arabidopsis/drug effects,embryology,genetics Arabidopsis Proteins/genetics,metabolism Benzyl Compounds Cytokinins/pharmacology Gene Expression Regulation, Plant/drug effects Indoleacetic Acids/pharmacology Kinetin/pharmacology Models, Biological Plant Roots/drug effects,embryology,genetics Plants, Genetically Modified Purines Reverse Transcriptase Polymerase Chain Reaction Xylem/genetics,metabolism
Chemicals
Arabidopsis Proteins Benzyl Compounds Cytokinins Indoleacetic Acids Purines Alkyl and Aryl Transferases adenylate isopentenyltransferase benzylaminopurine Kinetin
Authors & Affiliations
17 authors, click to expand affiliations / ORCID
Laplaze Laurent
Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité et Adaptation des Plantes Cultivées, Agro.M, Université Montpellier 2, Equipe Rhizogenèse, France. laplaze@mpl.ird.fr
Benkova Eva
Casimiro Ilda
Maes Lies
Vanneste Steffen
Swarup Ranjan
Weijers Dolf
Calvo Vanessa
Parizot Boris
Herrera-Rodriguez Maria Begoña
Offringa Remko
Graham Neil
Doumas Patrick
Friml Jiri
Bogusz Didier
Beeckman Tom
Bennett Malcolm
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43 references, click to expand
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.107.055863
Email
laplaze@mpl.ird.fr
Published
2007-12-00
Epub
2007-00-07
Pages
3889-900
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2217640
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/D019613/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · REI20541 · United Kingdom
External Links
PubMed source DOI: 10.1105/tpc.107.055863 PMC Full Text View journal details
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