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PMID: 12837947 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The CUP-SHAPED COTYLEDON3 gene is required for boundary and shoot meristem formation in Arabidopsis.

The Plant cell ·Vol. 15 ·No. 7 ·2003-07-00 ·Pages 1563-77

Vroemen CW, Mordhorst AP, Albrecht C, Kwaaitaal MA, de Vries SC

Abstract

From an enhancer trap screen for genes expressed in Arabidopsis embryos, we identified a gene expressed from the octant stage onward in the boundary between the two presumptive cotyledons and in a variety of postembryonic organ and meristem boundaries. This gene, CUP-SHAPED COTYLEDON3 (CUC3), encodes a putative NAC-domain transcription factor that is homologous with CUC1 and CUC2. Analysis of a CUC3 hypomorph and a putative cuc3 null mutant indicates that CUC3 function is partially redundant with that of CUC1 and CUC2 in the establishment of the cotyledon boundary and the shoot meristem, thus revealing an even higher degree of redundancy in this class of genes than was thought previously. The CUC3 expression pattern, the cuc3 phenotypes, and CUC3 expression in a series of shoot meristem mutants and transgenes suggest a primary role for CUC3 in the establishment of boundaries that contain cells with low proliferation and/or differentiation rates. The CUC-mediated establishment of such boundaries may be essential for the initiation of shoot meristems.

MeSH Terms
Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Base Sequence Cloning, Molecular DNA, Plant/chemistry,genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Glucuronidase/genetics,metabolism Homeodomain Proteins/genetics,metabolism In Situ Hybridization Meristem/genetics,growth & development,metabolism Molecular Sequence Data Mutation Phenotype Phylogeny Plant Shoots/genetics,growth & development,metabolism Seeds/genetics,growth & development,metabolism Sequence Analysis, DNA Transcription Factors/genetics,metabolism
Chemicals
Arabidopsis Proteins CUC1 protein, Arabidopsis CUC2 protein, Arabidopsis CUC3 protein, Arabidopsis DNA, Plant Homeodomain Proteins KNAT1 protein, Arabidopsis Transcription Factors Glucuronidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vroemen Casper W
Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University, Wageningen, The Netherlands. casper.vroemen@wur.nl
Mordhorst Andreas P
Albrecht Cathy
Kwaaitaal Mark A C J
de Vries Sacco C
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.012203
Email
casper.vroemen@wur.nl
Published
2003-07-00
Pages
1563-77
Language
English
Region
England
NLM ID
9208688
PMCID
PMC165401
Subset
IM
Databases
GENBANK
AF543194
External Links
PubMed source DOI: 10.1105/tpc.012203 PMC Full Text View journal details
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