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PMID: 9426607 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Characterization of the cyclophilin gene family of Arabidopsis thaliana and phylogenetic analysis of known cyclophilin proteins.

Plant molecular biology ·Vol. 35 ·No. 6 ·1997-12-00 ·Pages 873-92

Chou IT, Gasser CS

Abstract

We have isolated four members of the Arabidopsis cyclophilin (CyP) gene family, designated ROC1 to ROC4 (rotamase CyP). Deduced peptides of ROC1, 2 and 3 are 75% to 91% identical to Brassica napus cytosolic CyP, contain no leader peptides and include a conserved seven amino-acid insertion relative to mammalian cytosolic CyPs. Two other Arabidopsis CyPs, ROC5 (43H1; ATCYP1) and ROC6 (ATCYP2), share these features. ROC1, ROC2, ROC3 and ROC5 are expressed in all tested organs of light-grown plants. ROC2 and ROC5 show elevated expression in flowers. Expression of ROC1, ROC2, and ROC3 decreases in darkness and these genes also exhibit small elevations in expression upon wouding. The five Arabidopsis genes encoding putative cytosolic CyPs (ROC1, 2, 3, 5 and 6) contain no introns. In contrast, ROC4, which encodes a chloroplast stromal CyP, is interrupted by six introns. ROC4 is not expressed in roots, and is strongly induced by light. Phylogenetic trees of all known CyPs and CyP-related proteins provide evidence of possible horizontal transfer of CyP genes between prokaryotes and eukaryotes and of a possible polyphyletic origin of these proteins within eukaryotes. These trees also show significant grouping of eukaryotic CyPs on the basis of subcellular localization and structure. Mitochondrial CyPs are closely related to cytosolic CyPs of the source organism, but endoplasmic reticulum CyPs form separate clades. Known plant CyPs fall into three clades, one including the majority of higher-plant cytosolic CyPs, one including only ROC2 and a related rice CyP, and one including only chlorplast CyPs.

MeSH Terms
Amino Acid Sequence Animals Arabidopsis/enzymology,genetics Gene Dosage Gene Expression Regulation, Plant Genes, Plant Humans Light Molecular Sequence Data Multigene Family Peptidylprolyl Isomerase/biosynthesis,genetics Phylogeny Plant Proteins/biosynthesis,genetics RNA, Messenger/metabolism Stress, Physiological/genetics,metabolism
Chemicals
Plant Proteins RNA, Messenger Peptidylprolyl Isomerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chou I T
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.
Gasser C S
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005930024796
Published
1997-12-00
Pages
873-92
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
U40399, U40400, U42724
External Links
PubMed source DOI: 10.1023/a:1005930024796 View journal details
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