Home LiteratureArticle Details
PMID: 1938888 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12.

Journal of bacteriology ·Vol. 173 ·No. 21 ·1991-11-00 ·Pages 6837-43

Lloyd RG, Sharples GJ

Abstract

The nucleotide sequence of the Escherichia coli K-12 recG gene was determined. recG was identified as an open reading frame located between the spoT operon and the convergent gltS gene. It encodes a polypeptide of 693 amino acids which was identified as a 76-kDa protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after it was labeled with [35S]methionine in maxicells. The sequence determined revealed no obvious promoter. Synthesis of RecG by plasmids carrying the intact gene varied with the orientation of the insert relative to the vector promoter and with the extent of upstream spoT operon sequence included in the construction. It is concluded that recG is the fourth and last gene in the spoT operon, although a possible promoter for independent transcription of spoU and recG was identified near the end of the spoT gene. The primary sequence of RecG revealed that it is related to proteins that act as helicases and has a well-conserved motif identified with ATP binding.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA Helicases/genetics,metabolism DNA, Bacterial/metabolism Escherichia coli/genetics Escherichia coli Proteins Genes, Bacterial Molecular Sequence Data Operon Plasmids Restriction Mapping Sequence Alignment
Chemicals
Bacterial Proteins DNA, Bacterial Escherichia coli Proteins RecG protein, E coli DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lloyd R G
Department of Genetics, Queens Medical Centre, University of Nottingham, United Kingdom.
Sharples G J
References (46)
46 references, click to expand
  1. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA.
    Genetics. 1966 Jun;53(6):1119-36 PMID: 5335128
  2. Molecular cloning of the uvrD gene of Escherichia coli that controls ultraviolet sensitivity and spontaneous mutation frequency.
    Mol Gen Genet. 1981;184(2):191-9 PMID: 6276691
  3. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  4. Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype.
    J Bacteriol. 1974 Oct;120(1):407-15 PMID: 4607624
  5. The genetic location of the sbcA gene of Escherichia coli.
    Mol Gen Genet. 1974;134(2):157-71 PMID: 4617161
  6. Induction of protein synthesis in Escherichia coli following UV- or gamma-irradiation, mitomycin C treatment or tif Expression.
    Mol Gen Genet. 1977 Feb 28;151(1):57-67 PMID: 325368
  7. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  8. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  9. A unified mechanism for the nuclease and unwinding activities of the recBC enzyme of Escherichia coli.
    J Biol Chem. 1982 Mar 10;257(5):2641-8 PMID: 7037768
  10. Cloning of the uvrD gene of E. coli and identification of the product.
    Gene. 1982 Oct;19(3):285-95 PMID: 6218013
  11. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.
    Microbiol Rev. 1984 Mar;48(1):60-93 PMID: 6371470
  12. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  13. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  14. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  15. Cloning of eukaryotic protein synthesis initiation factor genes: isolation and characterization of cDNA clones encoding factor eIF-4A.
    Nucleic Acids Res. 1985 Oct 11;13(19):6867-80 PMID: 3840589
  16. Overlapping arrangement of the recF and dnaN operons of Escherichia coli; positive and negative control sequences.
    Gene. 1986;43(3):183-96 PMID: 3527871
  17. Analysis of E. coli promoter sequences.
    Nucleic Acids Res. 1987 Mar 11;15(5):2343-61 PMID: 3550697
  18. Consensus topography in the ATP binding site of the simian virus 40 and polyomavirus large tumor antigens.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):4026-30 PMID: 3035562
  19. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  20. Homologous recombination in procaryotes.
    Microbiol Rev. 1988 Mar;52(1):1-28 PMID: 3280962
  21. Nuclear protein with sequence homology to translation initiation factor eIF-4A.
    Nature. 1988 Apr 21;332(6166):736-8 PMID: 2451786
  22. A conserved NTP-motif in putative helicases.
    Nature. 1988 May 5;333(6168):22 PMID: 2834648
  23. A new superfamily of replicative proteins.
    Nature. 1988 May 5;333(6168):22-3 PMID: 3362205
  24. Transcriptional organization of the dnaN and recF genes of Escherichia coli K-12.
    J Biol Chem. 1988 Aug 25;263(24):12109-14 PMID: 2841344
  25. rpoZ, encoding the omega subunit of Escherichia coli RNA polymerase, is in the same operon as spoT.
    J Bacteriol. 1989 Mar;171(3):1271-7 PMID: 2646273
  26. Molecular analysis of the Escherichia coli recO gene.
    J Bacteriol. 1989 Jul;171(7):3641-9 PMID: 2544549
  27. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.
    Nucleic Acids Res. 1989 Jun 26;17(12):4713-30 PMID: 2546125
  28. Characterization of the spoT gene of Escherichia coli.
    J Biol Chem. 1989 Sep 5;264(25):15074-82 PMID: 2549050
  29. Molecular cloning and DNA sequence analysis of Escherichia coli priA, the gene encoding the primosomal protein replication factor Y.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4615-9 PMID: 2162049
  30. The priA gene encoding the primosomal replicative n' protein of Escherichia coli.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4620-4 PMID: 2162050
  31. Escherichia coli RecQ protein is a DNA helicase.
    Proc Natl Acad Sci U S A. 1990 Jul;87(14):5363-7 PMID: 2164680
  32. DNA helicases.
    Annu Rev Biochem. 1990;59:289-329 PMID: 2165383
  33. Further tests of a recombination model in which chi removes the RecD subunit from the RecBCD enzyme of Escherichia coli.
    Genetics. 1990 Nov;126(3):519-33 PMID: 2249753
  34. Nucleotide sequence of the Escherichia coli recJ chromosomal region and construction of recJ-overexpression plasmids.
    J Bacteriol. 1991 Jan;173(1):353-64 PMID: 1987126
  35. Genetic analysis of the recG locus of Escherichia coli K-12 and of its role in recombination and DNA repair.
    J Bacteriol. 1991 Feb;173(3):1004-11 PMID: 1846849
  36. Helical interactions in homologous pairing and strand exchange driven by RecA protein.
    J Biol Chem. 1991 Mar 25;266(9):5355-8 PMID: 2005084
  37. Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.
    J Biol Chem. 1991 Mar 25;266(9):5980-90 PMID: 2005134
  38. Evidence of abortive recombination in ruv mutants of Escherichia coli K12.
    Mol Gen Genet. 1991 Feb;225(2):266-72 PMID: 2005868
  39. Characterization of the Escherichia coli K12 gltS glutamate permease gene.
    Mol Gen Genet. 1991 Mar;225(3):379-86 PMID: 2017136
  40. Resolution of Holliday junctions in vitro requires the Escherichia coli ruvC gene product.
    Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6063-7 PMID: 1829835
  41. Conjugational recombination in resolvase-deficient ruvC mutants of Escherichia coli K-12 depends on recG.
    J Bacteriol. 1991 Sep;173(17):5414-8 PMID: 1653210
  42. DNA repair in Escherichia coli: identification of the uvrD gene product.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5616-20 PMID: 6291053
  43. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  44. Modulation of the action of the recBC enzyme of Escherichia coli K-12 by Ca2+.
    J Biol Chem. 1979 Sep 10;254(17):8646-52 PMID: 157358
  45. Unwinding and rewinding of DNA by the RecBC enzyme.
    Cell. 1980 Nov;22(2 Pt 2):447-57 PMID: 6256081
  46. Genetic recombination in Escherichia coli. IV. Isolation and characterization of recombination-deficiency mutants of Escherichia coli K12.
    Mutat Res. 1971 Sep;13(1):9-17 PMID: 4938964
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.21.6837-6843.1991
Published
1991-11-00
Pages
6837-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209035
Subset
IM
Grants
Wellcome Trust · United Kingdom
Databases
GENBANK
M63251, M63252, M63253, M63254, M63255, S59274, S60896, S61085, S61740, X59550
External Links
PubMed source DOI: 10.1128/jb.173.21.6837-6843.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2