Home LiteratureArticle Details
PMID: 19382792 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

FvVE1 regulates biosynthesis of the mycotoxins fumonisins and fusarins in Fusarium verticillioides.

Journal of agricultural and food chemistry ·Vol. 57 ·No. 11 ·2009-06-10 ·Pages 5089-94

Myung K, Li S, Butchko RA, Busman M, Proctor RH, Abbas HK, Calvo AM

Abstract

The veA gene positively regulates sterigmatocystin production in Aspergillus nidulans and aflatoxin production in Aspergillus parasiticus and Aspergillus flavus . Whether veA homologues have a role in regulating secondary metabolism in other fungal genera is unknown. In this study, we examined the role of the veA homologue, FvVE1, on the production of two mycotoxin families, fumonisins and fusarins, in the important corn pathogen Fusarium verticillioides . We found that FvVE1 deletion completely suppressed fumonisin production on two natural substrates, corn and rice. Furthermore, our results revealed that FvVE1 is necessary for the expression of the pathway-specific regulatory gene FUM21 and structural genes in the fumonisin biosynthetic gene (FUM) cluster. FvVE1 deletion also blocked production of fusarins. The effects of FvVE1 deletion on the production of these toxins were found to be the same in two separate mating types. Our results strongly suggest that FvVE1 plays an important role in regulating mycotoxin production in F. verticillioides .

MeSH Terms
Fumonisins/metabolism Fungal Proteins/genetics,metabolism Fusarium/genetics,metabolism Gene Expression Regulation, Fungal Mycotoxins/biosynthesis,genetics
Chemicals
Fumonisins Fungal Proteins Mycotoxins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Myung Kyung
Department of Biological Sciences, Northern Illinois University, Dekalb, Illinois 60115, USA.
Li Shaojie
Butchko Robert A E
Busman Mark
Proctor Robert H
Abbas Hamed K
Calvo Ana M
References (32)
32 references, click to expand
  1. FvVE1 regulates filamentous growth, the ratio of microconidia to macroconidia and cell wall formation in Fusarium verticillioides.
    Mol Microbiol. 2006 Dec;62(5):1418-32 PMID: 17054442
  2. Characterization of a transcriptional activator controlling trichothecene toxin biosynthesis.
    Fungal Genet Biol. 1999 Apr;26(3):224-35 PMID: 10361036
  3. veA is required for toxin and sclerotial production in Aspergillus parasiticus.
    Appl Environ Microbiol. 2004 Aug;70(8):4733-9 PMID: 15294809
  4. The VeA regulatory system and its role in morphological and chemical development in fungi.
    Fungal Genet Biol. 2008 Jul;45(7):1053-61 PMID: 18457967
  5. PAC1, a pH-regulatory gene from Fusarium verticillioides.
    Appl Environ Microbiol. 2003 Sep;69(9):5222-7 PMID: 12957906
  6. The White Collar protein WcoA of Fusarium fujikuroi is not essential for photocarotenogenesis, but is involved in the regulation of secondary metabolism and conidiation.
    Fungal Genet Biol. 2008 May;45(5):705-18 PMID: 18203635
  7. Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis.
    Science. 1999 May 21;284(5418):1368-72 PMID: 10334994
  8. Analytical determination of fumonisins and other metabolites produced by Fusarium moniliforme and related species on corn.
    Adv Exp Med Biol. 1996;392:57-64 PMID: 8850605
  9. Mycotoxins produced from fungi isolated from foodstuffs and soil: comparison of toxicity in fibroblasts and rat feeding tests.
    Appl Environ Microbiol. 1984 Sep;48(3):654-61 PMID: 6541890
  10. Fumonisins--novel mycotoxins with cancer-promoting activity produced by Fusarium moniliforme.
    Appl Environ Microbiol. 1988 Jul;54(7):1806-11 PMID: 2901247
  11. A mutagenic metabolite produced by Fusarium moniliforme isolated from Linxian county, China.
    Carcinogenesis. 1985 Jun;6(6):903-5 PMID: 4006077
  12. Characterization of four clustered and coregulated genes associated with fumonisin biosynthesis in Fusarium verticillioides.
    Fungal Genet Biol. 2001 Dec;34(3):155-65 PMID: 11728154
  13. Fumonisins, mycotoxins produced by fusarium species: biology, chemistry, and significance.
    Annu Rev Phytopathol. 1993;31:233-52 PMID: 18643768
  14. The expression of sterigmatocystin and penicillin genes in Aspergillus nidulans is controlled by veA, a gene required for sexual development.
    Eukaryot Cell. 2003 Dec;2(6):1178-86 PMID: 14665453
  15. Fck1, a C-type cyclin-dependent kinase, interacts with Fcc1 to regulate development and secondary metabolism in Fusarium verticillioides.
    Fungal Genet Biol. 2006 Mar;43(3):146-54 PMID: 16504555
  16. Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus.
    Appl Environ Microbiol. 1993 Jan;59(1):156-62 PMID: 8439147
  17. Identification of a gene cluster responsible for the biosynthesis of aurofusarin in the Fusarium graminearum species complex.
    Fungal Genet Biol. 2005 May;42(5):420-33 PMID: 15809006
  18. Determining the biosynthetic sequence in the early steps of the fumonisin pathway by use of three gene-disruption mutants of Fusarium verticillioides.
    J Agric Food Chem. 2004 May 19;52(10):2855-60 PMID: 15137825
  19. Mycotoxin production by Fusarium proliferatum isolates from rice with Fusarium sheath rot disease.
    Mycopathologia. 1999;147(2):97-104 PMID: 10967968
  20. Involvement of ZFR1 of Fusarium verticillioides in kernel colonization and the regulation of FST1, a putative sugar transporter gene required for fumonisin biosynthesis on maize kernels.
    Mol Plant Pathol. 2008 Mar;9(2):203-11 PMID: 18705852
  21. Fumonisins and fumonisin analogs as inhibitors of ceramide synthase and inducers of apoptosis.
    Biochim Biophys Acta. 2002 Dec 30;1585(2-3):188-92 PMID: 12531553
  22. Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1.
    Appl Environ Microbiol. 2004 May;70(5):2653-9 PMID: 15128515
  23. Production of cyclopiazonic acid, aflatrem, and aflatoxin by Aspergillus flavus is regulated by veA, a gene necessary for sclerotial formation.
    Appl Microbiol Biotechnol. 2007 Jan;73(5):1158-68 PMID: 16988822
  24. The Aspergillus parasiticus protein AFLJ interacts with the aflatoxin pathway-specific regulator AFLR.
    Mol Genet Genomics. 2003 Mar;268(6):711-9 PMID: 12655397
  25. Molecular biology of Fusarium mycotoxins.
    Int J Food Microbiol. 2007 Oct 20;119(1-2):47-50 PMID: 17707105
  26. Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis.
    Fungal Genet Biol. 2003 Mar;38(2):237-49 PMID: 12620260
  27. Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus.
    Curr Genet. 1996 May;29(6):549-55 PMID: 8662194
  28. Production of fumonisin analogs by Fusarium species.
    Appl Environ Microbiol. 2002 May;68(5):2101-5 PMID: 11976077
  29. Regulation of fumonisin B(1) biosynthesis and conidiation in Fusarium verticillioides by a cyclin-like (C-type) gene, FCC1.
    Appl Environ Microbiol. 2001 Apr;67(4):1607-12 PMID: 11282612
  30. Functional analysis of the polyketide synthase genes in the filamentous fungus Gibberella zeae (anamorph Fusarium graminearum).
    Eukaryot Cell. 2005 Nov;4(11):1926-33 PMID: 16278459
  31. Fusarin C biosynthesis in Fusarium moniliforme and Fusarium venenatum.
    Chembiochem. 2004 Sep 6;5(9):1196-1203 PMID: 15368570
  32. The Fusarium verticillioides FUM gene cluster encodes a Zn(II)2Cys6 protein that affects FUM gene expression and fumonisin production.
    Eukaryot Cell. 2007 Jul;6(7):1210-8 PMID: 17483290
Article Info
Journal
Journal of agricultural and food chemistry
Abbr.
J Agric Food Chem
ISSN
1520-5118
DOI
10.1021/jf900783u
Published
2009-06-10
Pages
5089-94
Language
English
Region
United States
NLM ID
0374755
PMCID
PMC2692565
Subset
IM
Grants
NIGMS NIH HHS · R15 GM074267 · United States
NIGMS NIH HHS · R15 GM074267-01A1 · United States
External Links
PubMed source DOI: 10.1021/jf900783u PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2