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PMID: 19377470 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Myosin-dependent targeting of transmembrane proteins to neuronal dendrites.

Nature neuroscience ·Vol. 12 ·No. 5 ·2009-05-00 ·Pages 568-76

Lewis TL, Mao T, Svoboda K, Arnold DB

Abstract

The distinct electrical properties of axonal and dendritic membranes are largely a result of specific transport of vesicle-bound membrane proteins to each compartment. How this specificity arises is unclear because kinesin motors that transport vesicles cannot autonomously distinguish dendritically projecting microtubules from those projecting axonally. We hypothesized that interaction with a second motor might enable vesicles containing dendritic proteins to preferentially associate with dendritically projecting microtubules and avoid those that project to the axon. Here we show that in rat cortical neurons, localization of several distinct transmembrane proteins to dendrites is dependent on specific myosin motors and an intact actin network. Moreover, fusion with a myosin-binding domain from Melanophilin targeted Channelrhodopsin-2 specifically to the somatodendritic compartment of neurons in mice in vivo. Together, our results suggest that dendritic transmembrane proteins direct the vesicles in which they are transported to avoid the axonal compartment through interaction with myosin motors.

MeSH Terms
Actin Cytoskeleton/metabolism,ultrastructure Adaptor Proteins, Signal Transducing/chemistry,metabolism Animals Cell Compartmentation/physiology Cell Polarity/physiology Cerebral Cortex/metabolism,ultrastructure Channelrhodopsins Cytoskeleton/metabolism,ultrastructure Dendrites/metabolism,ultrastructure Membrane Proteins/metabolism Mice Microtubules/metabolism,ultrastructure Molecular Motor Proteins/metabolism Myosins/metabolism Protein Transport/physiology Rats Recombinant Fusion Proteins/metabolism Transport Vesicles/metabolism,ultrastructure
Chemicals
Adaptor Proteins, Signal Transducing Channelrhodopsins Membrane Proteins Mlph protein, mouse Molecular Motor Proteins Recombinant Fusion Proteins Myosins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lewis Tommy L
Department of Biological Sciences, University of Southern California, Los Angeles, USA.
Mao Tianyi
Svoboda Karel
Arnold Don B
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Article Info
Journal
Nature neuroscience
Abbr.
Nat Neurosci
ISSN
1546-1726
DOI
10.1038/nn.2318
Published
2009-05-00
Epub
2009-00-19
Pages
568-76
Language
English
Region
United States
NLM ID
9809671
PMCID
PMC2937175
Subset
IM
Grants
NIGMS NIH HHS · R01 GM083898-02 · United States
NIMH NIH HHS · K02 MH071439 · United States
NINDS NIH HHS · R01 NS041963-06 · United States
NINDS NIH HHS · NS-041963 · United States
Howard Hughes Medical Institute · United States
NIMH NIH HHS · MH-071439 · United States
NINDS NIH HHS · F32 NS080464 · United States
NIGMS NIH HHS · R01 GM083898 · United States
NINDS NIH HHS · R01 NS041963 · United States
NIMH NIH HHS · K02 MH071439-05 · United States
External Links
PubMed source DOI: 10.1038/nn.2318 PMC Full Text View journal details
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