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PMID: 1930153 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The identification and purification of the heterotrimeric GTP-binding protein from squid (Loligo forbesi) photoreceptors.

The Biochemical journal ·Vol. 279 ( Pt 1) ·1991-10-01 ·Pages 323-6

Pottinger JD, Ryba NJ, Keen JN, Findlay JB

Abstract

The principal GTP-binding protein (G-protein) from squid (Loligo forbesi) photoreceptor membranes has been identified by amino acid sequencing. The heterotrimeric protein was purified by detergent solubilization and ion-exchange chromatography. The amino acid sequence of the G-protein alpha-subunit (G-alpha) indicates that this subunit is closely related to the recently characterized Gq subgroup, whereas the G-gamma subunit varies widely in sequence from other homologues.

MeSH Terms
Amino Acid Sequence Animals Chromatography, Ion Exchange Cyanogen Bromide Decapodiformes Detergents Electrophoresis, Polyacrylamide Gel GTP-Binding Proteins/chemistry,isolation & purification Iodobenzoates/chemistry Molecular Sequence Data Photoreceptor Cells/chemistry Sequence Homology, Nucleic Acid Urea/chemistry
Chemicals
Detergents Iodobenzoates Urea GTP-Binding Proteins Cyanogen Bromide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pottinger J D
Department of Biochemistry and Molecular Biology, University of Leeds, U.K.
Ryba N J
Keen J N
Findlay J B
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31 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2790323
Published
1991-10-01
Pages
323-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1151585
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2790323 PMC Full Text View journal details
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