Purification of hepatitis A virus from chimpanzee stools.

Journal of virology ·Vol. 22 ·No. 1 ·1977-04-00 ·Pages 228-31

Bradley DW, Hornbeck CL, Cook EH, Maynard JE

Abstract

Hepatitis A virus antigen was purified from early acute-phase chimpanzee stools by a rapid three-step procedure using 7% polyethylene glycol precipitation, CsCl banding, and Sepharose 2B column chromatography. Electron microscopic examination of the hepatitis A virus entigen preparation revealed highly purified hepatitis A virus particles.

MeSH Terms

Animals Antigens, Viral/isolation & purification Feces/microbiology Hepatovirus/immunology,isolation & purification,ultrastructure Pan troglodytes Radioimmunoassay

Chemicals

Antigens, Viral

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bradley D W

Hornbeck C L

Cook E H

Maynard J E

References (12)

12 references, click to expand

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J Infect Dis. 1975 Feb;131(2):167-71 PMID: 1117190
Preliminary studies of hepatitis A in chimpanzees.
J Infect Dis. 1975 Feb;131(2):194-7 PMID: 1117191
CsCl banding of hepatitis A-associated virus-like particles.
J Infect Dis. 1975 Mar;131(3):304-6 PMID: 1127258
Physical, chemical and morphologic dimensions of human hepatitis A virus strain CR326 (38578).
Proc Soc Exp Biol Med. 1975 Feb;148(2):532-9 PMID: 164674
Experimental infection of chimpanzees with hepatitis A virus.
J Infect Dis. 1975 Nov;132(5):532-45 PMID: 171320
Hepatitis in marmosets.
Am J Med Sci. 1975 Jul-Aug;270(1):73-80 PMID: 171953
Review of infectivity studies in nonhuman primates with virus-like particles associated with MS-1 hepatitis.
Am J Med Sci. 1975 Jul-Aug;270(1):81-5 PMID: 171954
A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody.
J Immunol. 1976 Feb;116(2):349-56 PMID: 175117
Isolation and characterization of hepatitis A virus.
Am J Clin Pathol. 1976 May;65(5 Suppl):876-89 PMID: 218442
Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness.
Science. 1973 Dec 7;182(4116):1026-8 PMID: 4356028
Buoyant density of the hepatitis A virus-like particle in cesium chloride.
J Virol. 1974 Jun;13(6):1412-4 PMID: 4833615
Detection of hepatitis A viral antigen by radioimmunoassay.
J Immunol. 1975 Nov;115(5):1464-6 PMID: 51893

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.22.1.228-231.1977

Published

1977-04-00

Pages

228-31

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515704

Subset

External Links

PubMed source DOI: 10.1128/JVI.22.1.228-231.1977 PMC Full Text View journal details

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Purification of hepatitis A virus from chimpanzee stools.

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