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PMID: 1922024 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation and a possible stage-specific function of Oct-2 during pre-B-cell differentiation.

Molecular and cellular biology ·Vol. 11 ·No. 10 ·1991-10-00 ·Pages 4885-94

Miller CL, Feldhaus AL, Rooney JW, Rhodes LD, Sibley CH, Singh H

Abstract

The Oct-2 gene appears to encode a developmental regulator of immunoglobulin gene transcription. We demonstrate that the Oct-2 gene is expressed at low levels in a variety of transformed pre-B-cell lines and is induced specifically in these cells by lipopolysaccharide signalling. This work extends an earlier observation in the pre-B-cell line 70Z/3 and therefore suggests that the inducible expression of the Oct-2 gene, like that of the kappa gene, is a characteristic feature of the pre-B stage of B-cell development. In 70Z/3 cells, the lymphokine interleukin-1 also induces the expression of the Oct-2 and kappa loci. Interestingly, expression of the Oct-2 gene is rapidly induced at the transcriptional level and may not require de novo protein synthesis. Since the changes in the activity of the Oct-2 locus completely correlate with the changes of the activity of the kappa locus, the two genes may be transcriptionally regulated by a common trans-acting factor. In 70Z/3 cells, transforming growth factor beta, an inhibitor of kappa-gene induction, blocks the upregulation of Oct-2 but not the activation of NF-kappa B. These results suggest that the combinatorial action of increased levels of Oct-2 and activated NF-kappa B may be necessary for the proper stage-specific expression of the kappa locus.

Related Genes
Oct-2
MeSH Terms
Animals B-Lymphocytes/cytology,physiology Blotting, Northern Blotting, Western Cell Differentiation/physiology Cell Line Cells, Cultured DNA-Binding Proteins Gene Expression Regulation/drug effects Immunoglobulin kappa-Chains/genetics,physiology Interleukin-1/pharmacology Lipopolysaccharides Mice Octamer Transcription Factor-2 Transcription Factors/genetics,physiology Transcriptional Activation Transforming Growth Factor beta/pharmacology
Chemicals
DNA-Binding Proteins Immunoglobulin kappa-Chains Interleukin-1 Lipopolysaccharides Octamer Transcription Factor-2 Pou2f2 protein, mouse Transcription Factors Transforming Growth Factor beta
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Miller C L
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Feldhaus A L
Rooney J W
Rhodes L D
Sibley C H
Singh H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.10.4885-4894.1991
Published
1991-10-00
Pages
4885-94
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361457
Subset
IM
Grants
NIGMS NIH HHS · GM07183 · United States
NIGMS NIH HHS · GM07270 · United States
NIGMS NIH HHS · GM42508 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.10.4885-4894.1991 PMC Full Text View journal details
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