Home LiteratureArticle Details
PMID: 1921973 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 2.6 kb intron separates the signal peptide coding sequence of an anther-specific protein from the rest of the gene in sunflower.

Molecular & general genetics : MGG ·Vol. 229 ·No. 2 ·1991-10-00 ·Pages 238-44

Domon C, Evrard JL, Pillay DT, Steinmetz A

Abstract

We have isolated and sequenced an anther-specific gene from sunflower which encodes an 800-nucleotide transcript detectable in the peripheral anther cells. It contains an intron of 2615 bp, which separates the first exon (77 bp) coding for a putative signal peptide of 21 amino acids, from the second exon (563 bp) coding for a 100 amino acid polypeptide. The 5' and 3' untranslated regions comprise respectively 13 and 264 bp. The SF2 gene is present in the sunflower genome in several copies, all or most of which contain a closely related intron.

Related Genes
SF1
MeSH Terms
Amino Acid Sequence Bacteriophages/genetics Base Sequence Blotting, Southern Cell Nucleus/chemistry DNA/genetics Escherichia coli/genetics Genes, Bacterial Genes, Viral Helianthus/chemistry Introns Molecular Sequence Data Plant Proteins/genetics Plasmids Protein Sorting Signals/genetics Restriction Mapping Transcription, Genetic
Chemicals
Plant Proteins Protein Sorting Signals DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Domon C
Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Evrard J L
Pillay D T
Steinmetz A
References (30)
30 references, click to expand
  1. Isolation and expression of an anther-specific gene from tomato.
    Mol Gen Genet. 1989 Jun;217(2-3):240-5 PMID: 2770694
  2. The rice phytochrome gene: structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region.
    Plant Cell. 1989 Mar;1(3):351-60 PMID: 2535506
  3. Gametophytic and sporophytic expression of anther-specific genes in developing tomato anthers.
    Plant Cell. 1989 Jul;1(7):727-36 PMID: 2535521
  4. Double stranded DNA sequencing as a choice for DNA sequencing.
    Nucleic Acids Res. 1988 Feb 11;16(3):1220 PMID: 3344219
  5. The genes coding for subunit 3 of NADH dehydrogenase and for ribosomal protein S12 are present in the wheat and maize mitochondrial genomes and are co-transcribed.
    Mol Gen Genet. 1988 Dec;215(1):118-27 PMID: 2853827
  6. Regulation of structural gene expression in tobacco.
    Cell. 1980 Apr;19(4):935-46 PMID: 7379124
  7. The conserved CAAGAAAGA spacer sequence is an essential element for the formation of 3' termini of the sea urchin H3 histone mRNA by RNA processing.
    EMBO J. 1985 Feb;4(2):481-9 PMID: 2410259
  8. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  9. Anther-specific, developmentally regulated expression of genes encoding a new class of proline-rich proteins in sunflower.
    Plant Mol Biol. 1991 Feb;16(2):271-81 PMID: 1716499
  10. The nucleotide sequence of an anther-specific gene.
    Nucleic Acids Res. 1990 Jun 11;18(11):3403 PMID: 2356131
  11. Patterns of amino acids near signal-sequence cleavage sites.
    Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
  12. Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development.
    Plant Cell. 1990 Dec;2(12):1201-1224 PMID: 12354953
  13. Pollen- and anther-specific chi promoters from petunia: tandem promoter regulation of the chiA gene.
    Plant Cell. 1990 May;2(5):393-401 PMID: 2152165
  14. Poly(A) signals.
    Cell. 1991 Feb 22;64(4):671-4 PMID: 1671760
  15. Characterization of a pollen-specific cDNA clone from Zea mays and its expression.
    Plant Cell. 1989 Feb;1(2):173-9 PMID: 2535540
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. Organ-specific nuclear RNAs in tobacco.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2801-5 PMID: 6201864
  18. Identification and characterization of stamen- and tapetum-specific genes from tomato.
    Mol Gen Genet. 1990 Jun;222(1):9-16 PMID: 2233685
  19. Characterization of the glycinin gene family in soybean.
    Plant Cell. 1989 Mar;1(3):313-28 PMID: 2485233
  20. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  21. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  22. Nucleotide sequence of two anther-specific cDNAs from sunflower (Helianthus annuus L.).
    Plant Mol Biol. 1990 Oct;15(4):643-6 PMID: 2102380
  23. Genes expressed in the male gametophyte of flowering plants and their isolation.
    Plant Physiol. 1987 Feb;83(2):442-7 PMID: 16665265
  24. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  25. Characterization of a gene family abundantly expressed in Oenothera organensis pollen that shows sequence similarity to polygalacturonase.
    Plant Cell. 1990 Mar;2(3):263-74 PMID: 2152116
  26. A survey on intron and exon lengths.
    Nucleic Acids Res. 1988 Nov 11;16(21):9893-908 PMID: 3057449
  27. The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicing.
    Cell. 1989 Aug 11;58(3):473-83 PMID: 2758463
  28. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  29. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  30. Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers.
    Nucleic Acids Res. 1987 Mar 25;15(6):2677-98 PMID: 3031608
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1991-10-00
Pages
238-44
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
S55526, S55527, S55528, S55529, S55530, S55531, S55532, S60122, S60125, X62716
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com