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PMID: 19214217 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Arabidopsis CaM binding protein CBP60g contributes to MAMP-induced SA accumulation and is involved in disease resistance against Pseudomonas syringae.

PLoS pathogens ·Vol. 5 ·No. 2 ·2009-02-00 ·Pages e1000301

Wang L, Tsuda K, Sato M, Cohen JD, Katagiri F, Glazebrook J

Abstract

Salicylic acid (SA)-induced defense responses are important factors during effector triggered immunity and microbe-associated molecular pattern (MAMP)-induced immunity in plants. This article presents evidence that a member of the Arabidopsis CBP60 gene family, CBP60g, contributes to MAMP-triggered SA accumulation. CBP60g is inducible by both pathogen and MAMP treatments. Pseudomonas syringae growth is enhanced in cbp60g mutants. Expression profiles of a cbp60g mutant after MAMP treatment are similar to those of sid2 and pad4, suggesting a defect in SA signaling. Accordingly, cbp60g mutants accumulate less SA when treated with the MAMP flg22 or a P. syringae hrcC strain that activates MAMP signaling. MAMP-induced production of reactive oxygen species and callose deposition are unaffected in cbp60g mutants. CBP60g is a calmodulin-binding protein with a calmodulin-binding domain located near the N-terminus. Calmodulin binding is dependent on Ca(2+). Mutations in CBP60g that abolish calmodulin binding prevent complementation of the SA production and bacterial growth defects of cbp60g mutants, indicating that calmodulin binding is essential for the function of CBP60g in defense signaling. These studies show that CBP60g constitutes a Ca(2+) link between MAMP recognition and SA accumulation that is important for resistance to P. syringae.

MeSH Terms
Analysis of Variance Arabidopsis/genetics,immunology,metabolism,microbiology Arabidopsis Proteins/genetics,physiology Calmodulin/metabolism Calmodulin-Binding Proteins/genetics,physiology Cluster Analysis Gene Expression Profiling Glucans/metabolism Immunity, Innate Intramolecular Transferases/metabolism Mutagenesis, Insertional Mutagenesis, Site-Directed Oligonucleotide Array Sequence Analysis Plant Diseases/genetics,immunology,microbiology Pseudomonas syringae/physiology Reactive Oxygen Species/metabolism Salicylic Acid/metabolism Signal Transduction
Chemicals
Arabidopsis Proteins Calmodulin Calmodulin-Binding Proteins Glucans Reactive Oxygen Species callose Intramolecular Transferases isochorismate synthase Salicylic Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wang Lin
Department of Plant Biology, Microbial and Plant Genomics Institute, University of Minnesota, St Paul, Minnesota, United States of America.
Tsuda Kenichi
Sato Masanao
Cohen Jerry D
Katagiri Fumiaki
Glazebrook Jane
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Article Info
Journal
PLoS pathogens
Abbr.
PLoS Pathog
ISSN
1553-7374
DOI
10.1371/journal.ppat.1000301
Published
2009-02-00
Epub
2009-00-13
Pages
e1000301
Language
English
Region
United States
NLM ID
101238921
PMCID
PMC2633612
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.ppat.1000301 PMC Full Text View journal details
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