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PMID: 1920632 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Discontinuous sequence change of human immunodeficiency virus (HIV) type 1 env sequences in plasma viral and lymphocyte-associated proviral populations in vivo: implications for models of HIV pathogenesis.

Journal of virology ·Vol. 65 ·No. 11 ·1991-11-00 ·Pages 6266-76

Simmonds P, Zhang LQ, McOmish F, Balfe P, Ludlam CA, Brown AJ

Abstract

Sequence change in different hypervariable regions of the external membrane glycoprotein (gp120) of human immunodeficiency virus type 1 (HIV-1) was studied. Viral RNA associated with cell-free virus particles circulating in plasma and proviral DNA present in HIV-infected peripheral blood mononuclear cells (PBMCs) were extracted from blood samples of two currently asymptomatic hemophiliac patients over a 5-year period. HIV sequences were amplified by polymerase chain reaction to allow analysis in the V3, V4, and V5 hypervariable regions of gp120. Rapid sequence change, consisting of regular replacements by a succession of distinct viral populations, was found in both plasma virus and PBMC provirus populations. Significant differences between the frequencies of sequence variants in DNA and RNA populations within the same sample were observed, indicating that at any one time point, the predominant plasma virus variants were antigenically distinct from viruses encoded by HIV DNA sequences in PBMCs. How these findings contribute to current models of HIV pathogenesis is discussed.

Related Genes
env
MeSH Terms
Acquired Immunodeficiency Syndrome/blood,immunology,microbiology Amino Acid Sequence Base Sequence Biological Evolution Genes, env Genetic Variation HIV Envelope Protein gp120/genetics HIV-1/genetics,isolation & purification Hemophilia A/blood,complications,microbiology Humans Lymphocytes/microbiology Molecular Sequence Data Oligodeoxyribonucleotides Oligonucleotides, Antisense Phylogeny Polymerase Chain Reaction Proviruses/genetics,isolation & purification
Chemicals
HIV Envelope Protein gp120 Oligodeoxyribonucleotides Oligonucleotides, Antisense
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Simmonds P
Department of Medical Microbiology, University of Edinburgh, Scotland.
Zhang L Q
McOmish F
Balfe P
Ludlam C A
Brown A J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.11.6266-6276.1991
Published
1991-11-00
Pages
6266-76
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250328
Subset
IM
Databases
GENBANK
M77541, M77636
External Links
PubMed source DOI: 10.1128/JVI.65.11.6266-6276.1991 PMC Full Text View journal details
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