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PMID: 1918155 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Three-dimensional reconstruction of the 70S Escherichia coli ribosome in ice: the distribution of ribosomal RNA.

The Journal of cell biology ·Vol. 115 ·No. 3 ·1991-11-00 ·Pages 597-605

Frank J, Penczek P, Grassucci R, Srivastava S

Abstract

A reconstruction, at 40 A, of the Escherichia coli ribosome imaged by cryo-electron microscopy, obtained from 303 projections by a single-particle method of reconstruction, shows the two subunits with unprecedented clarity. In the interior of the subunits, a complex distribution of higher mass density is recognized, which is attributed to ribosomal RNA. The masses corresponding to the 16S and 23S components are linked in the region of the platform of the small subunit. Thus the topography of the rRNA regions responsible for protein synthesis can be described.

MeSH Terms
Computer Graphics Escherichia coli/ultrastructure Models, Structural RNA, Ribosomal/ultrastructure RNA, Ribosomal, 16S/ultrastructure RNA, Ribosomal, 23S/ultrastructure Ribosomes/ultrastructure
Chemicals
RNA, Ribosomal RNA, Ribosomal, 16S RNA, Ribosomal, 23S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Frank J
Wadsworth Center for Laboratories and Research, Albany, New York 12201-0509.
Penczek P
Grassucci R
Srivastava S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.115.3.597
Published
1991-11-00
Pages
597-605
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289182
Subset
IM
Grants
NIGMS NIH HHS · 1R01 GM29169 · United States
External Links
PubMed source DOI: 10.1083/jcb.115.3.597 PMC Full Text View journal details
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