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PMID: 6348775 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Association of 16S and 23S ribosomal RNAs to form a bimolecular complex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 16 ·1983-08-00 ·Pages 4875-8

Burma DP, Nag B, Tewari DS

Abstract

Association of the 30S and 50S subunits to generate the 70S ribosomes of Escherichia coli has long been known but the mechanism of this interaction remains obscure. Light-scattering studies indicate that naked 16S and 23S RNAs can also associate under conditions similar to those required for the assembly of ribosomes from the constituent RNAs and proteins. The RNA-RNA association also takes place in the presence of ethanol, which promotes folding of 16S and 23S RNAs into specific compact structures with the morphological features of 30S and 50S ribosomes, respectively. Equimolar amounts of the two RNAs are involved in the association. The formation of a stoichiometric complex was shown by light scattering, sucrose density gradient centrifugation, and composite polyacrylamide/agarose gel electrophoresis. The presence of the two species of RNA in the complex was also shown by gel electrophoresis. The association of naked 16S and 23S RNAs suggests that RNA-RNA interaction may play an important role in the association of 30S and 50S subunits.

MeSH Terms
Escherichia coli/metabolism Ethanol/pharmacology Kinetics Macromolecular Substances Molecular Weight Phosphorus Radioisotopes RNA, Ribosomal/metabolism Ribosomes/metabolism
Chemicals
Macromolecular Substances Phosphorus Radioisotopes RNA, Ribosomal Ethanol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Burma D P
Nag B
Tewari D S
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.16.4875
Published
1983-08-00
Pages
4875-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384149
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.80.16.4875 PMC Full Text View journal details
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