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PMID: 191389 Published · ppublish English Journal Article

Genetic analysis by chromosome-mediated gene transfer.

In vitro ·Vol. 12 ·No. 11 ·1976-11-00 ·Pages 777-86

McBride OW, Athwal RS

Abstract

A general method is presented for stable transfer of genetic information to eukaryotic cells, utilizing metaphase chromosomes as the vehicle. Recent progress, current problems and large areas of uncertainty in this field are reviewed; particular consideration is given to frequency of transfer, size of the transgenome, evidence of cotransfer of linked genes and serial chromosome transfer. A reasonable model for chromosome transfer is considered with respect to the available information, and various descrepancies are noted. The utility of this method for fine structural mapping, cloning small regions of the eukaryotic genome and other potential applications are discussed.

MeSH Terms
Animals Cells, Cultured Chromosomes Cricetinae DNA/analysis Genes Humans Mice Models, Theoretical Nucleic Acid Hybridization Peptides/pharmacology Phagocytosis Phosphotransferases/biosynthesis Thymidine Kinase/biosynthesis Transformation, Genetic
Chemicals
Peptides DNA Phosphotransferases Thymidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McBride O W
Athwal R S
References (33)
33 references, click to expand
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02835452
Published
1976-11-00
Pages
777-86
Language
English
Region
United States
NLM ID
0063733
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02835452
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