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PMID: 19061643 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A ncRNA modulates histone modification and mRNA induction in the yeast GAL gene cluster.

Molecular cell ·Vol. 32 ·No. 5 ·2008-12-05 ·Pages 685-95

Houseley J, Rubbi L, Grunstein M, Tollervey D, Vogelauer M

Abstract

The extensively studied yeast GAL1-10 gene cluster is tightly regulated by environmental sugar availability. Unexpectedly, under repressive conditions the 3' region of the GAL10 coding sequence is trimethylated by Set1 on histone H3 K4, normally characteristic of 5' regions of actively transcribed genes. This reflects transcription of a long noncoding RNA (GAL10-ncRNA) that is reciprocal to GAL1 and GAL10 mRNAs and driven by the DNA-binding protein Reb1. Point mutations in predicted Reb1-binding sites abolished Reb1 binding and ncRNA synthesis. The GAL10-ncRNA is transcribed approximately once every 50 min and targeted for degradation by the TRAMP and exosome complexes, resulting in low steady-state levels (approximately one molecule per 14 cells). GAL10-ncRNA transcription recruits the methyltransferase Set2 and histone deacetylation activities in cis, leading to stable changes in chromatin structure. These chromatin modifications act principally through the Rpd3S complex to aid glucose repression of GAL1-10 at physiologically relevant sugar concentrations.

MeSH Terms
Acetyltransferases/metabolism Chromatin/metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation, Fungal/drug effects Genes, Fungal Glucose/pharmacology Histones/metabolism Lysine/metabolism Methylation/drug effects Models, Biological Multigene Family Protein Processing, Post-Translational/drug effects RNA, Messenger/genetics,metabolism RNA, Untranslated/metabolism Repressor Proteins/metabolism Saccharomyces cerevisiae/drug effects,genetics Saccharomyces cerevisiae Proteins/genetics,metabolism Transcription Factors/metabolism Transcription, Genetic/drug effects
Chemicals
Chromatin DNA-Binding Proteins Histones REB1 protein, S cerevisiae RNA, Messenger RNA, Untranslated Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors Acetyltransferases Eaf3 protein, S cerevisiae Glucose Lysine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Houseley Jonathan
Wellcome Trust Centre for Cell Biology, Institute for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK.
Rubbi Liudmilla
Grunstein Michael
Tollervey David
Vogelauer Maria
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-4164
DOI
10.1016/j.molcel.2008.09.027
Published
2008-12-05
Pages
685-95
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC7610895
Subset
IM
Grants
NIGMS NIH HHS · GM23674 · United States
NIGMS NIH HHS · R01 GM023674 · United States
Wellcome Trust · MV37766 · United Kingdom
Wellcome Trust · United Kingdom
Wellcome Trust · 073397 · United Kingdom
NIGMS NIH HHS · R37 GM023674 · United States
External Links
PubMed source DOI: 10.1016/j.molcel.2008.09.027 PMC Full Text View journal details
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