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PMID: 19061637 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

COP1 and ELF3 control circadian function and photoperiodic flowering by regulating GI stability.

Molecular cell ·Vol. 32 ·No. 5 ·2008-12-05 ·Pages 617-30

Yu JW, Rubio V, Lee NY, Bai S, Lee SY, Kim SS, Liu L, Zhang Y, Irigoyen ML, Sullivan JA, Zhang Y, Lee I, Xie Q, Paek NC, Deng XW

Abstract

Seasonal changes in day length are perceived by plant photoreceptors and transmitted to the circadian clock to modulate developmental responses such as flowering time. Blue-light-sensing cryptochromes, the E3 ubiquitin-ligase COP1, and clock-associated proteins ELF3 and GI regulate this process, although the regulatory link between them is unclear. Here we present data showing that COP1 acts with ELF3 to mediate day length signaling from CRY2 to GI within the photoperiod flowering pathway. We found that COP1 and ELF3 interact in vivo and show that ELF3 allows COP1 to interact with GI in vivo, leading to GI degradation in planta. Accordingly, mutation of COP1 or ELF3 disturbs the pattern of GI cyclic accumulation. We propose a model in which ELF3 acts as a substrate adaptor, enabling COP1 to modulate light input signal to the circadian clock through targeted destabilization of GI.

MeSH Terms
Arabidopsis/genetics,physiology Arabidopsis Proteins/genetics,metabolism Circadian Rhythm/physiology Flowers/genetics,physiology Gene Expression Regulation, Plant Genes, Plant Models, Biological Mutation/genetics Photoperiod Proteasome Endopeptidase Complex/metabolism Protein Binding Protein Processing, Post-Translational Protein Stability Transcription Factors/genetics,metabolism Ubiquitin-Protein Ligases/metabolism Ubiquitination
Chemicals
Arabidopsis Proteins ELF3 protein, Arabidopsis GI protein, Arabidopsis Transcription Factors AT2G32950 protein, Arabidopsis Ubiquitin-Protein Ligases Proteasome Endopeptidase Complex
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Yu Jae-Woong
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
Rubio Vicente
Lee Na-Yeoun
Bai Sulan
Lee Sun-Young
Kim Sang-Sook
Liu Lijing
Zhang Yiyue
Irigoyen María Luisa
Sullivan James A
Zhang Yu
Lee Ilha
Xie Qi
Paek Nam-Chon
Deng Xing Wang
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-4164
DOI
10.1016/j.molcel.2008.09.026
Published
2008-12-05
Pages
617-30
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC2651194
Subset
IM
Grants
NIGMS NIH HHS · R37 GM047850-15 · United States
NIGMS NIH HHS · R37 GM047850-16 · United States
NIGMS NIH HHS · GM-47850 · United States
NIGMS NIH HHS · R01 GM047850 · United States
NIGMS NIH HHS · R37 GM047850-13 · United States
NIGMS NIH HHS · R37 GM047850-17 · United States
NIGMS NIH HHS · R37 GM047850 · United States
NIGMS NIH HHS · R37 GM047850-14 · United States
External Links
PubMed source DOI: 10.1016/j.molcel.2008.09.026 PMC Full Text View journal details
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