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PMID: 1901867 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Endocytosis and intracellular transport of the glycolipid-binding ligand Shiga toxin in polarized MDCK cells.

The Journal of cell biology ·Vol. 113 ·No. 3 ·1991-05-00 ·Pages 553-62

Sandvig K, Prydz K, Ryd M, van Deurs B

Abstract

The glycolipid-binding cytotoxin produced by Shigella dysenteriae 1, Shiga toxin, binds to MDCK cells (strain 1) only after treatment with short-chain fatty acids like butyric acid or with the tumor promoter 12-O-tetradecanoylphorbol 13-acetate. The induced binding sites were found to be functional with respect to endocytosis and translocation of toxin to the cytosol. Glycolipids that bind Shiga toxin appeared at both the apical and the basolateral surface of polarized MDCK cells grown on filters, and Shiga toxin was found to be endocytosed from both sides of the cells. This was demonstrated by EM of cells incubated with Shiga-HRP and by subcellular fractionation of cells incubated with 125I-labeled Shiga toxin. The data indicated that toxin molecules are endocytosed from coated pits, and that some internalized Shiga toxin is transported to the Golgi apparatus. Fractionation of polarized cells incubated with 125I-Shiga toxin showed that the transport of toxin to the Golgi apparatus was equally efficient from both poles of the cells. After 1-h incubation at 37 degrees C approximately 10% of the internalized toxin was found in the Golgi fractions. The results thus suggest that glycolipids can be efficiently transported to the Golgi apparatus from both sides of polarized MDCK cell monolayers.

MeSH Terms
Animals Bacterial Toxins/metabolism,toxicity Binding Sites Butyrates/pharmacology Butyric Acid Cell Line Coated Pits, Cell-Membrane/metabolism Endocytosis/drug effects Golgi Apparatus/metabolism Lysosomes/metabolism Shiga Toxins Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Bacterial Toxins Butyrates Shiga Toxins Butyric Acid Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sandvig K
Institute for Cancer Research, Norwegian Radium Hospital, Montebello, Oslo.
Prydz K
Ryd M
van Deurs B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.113.3.553
Published
1991-05-00
Pages
553-62
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288972
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.113.3.553 PMC Full Text View journal details
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