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PMID: 4150488 Published · ppublish English Journal Article

Analytical study of microsomes and isolated subcellular membranes from rat liver. I. Biochemical methods.

The Journal of cell biology ·Vol. 61 ·No. 1 ·1974-04-00 ·Pages 188-200

Beaufay H, Amar-Costesec A, Feytmans E, Thinès-Sempoux D, Wibo M, Robbi M, Berthet J

Abstract

The series introduced by this paper reports the results of a detailed analysis of the microsomal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cytochrome c reductase, NADPH cytochrome c reductase, cytochrome oxidase, catalase, aminopyrine demethylase, cytochromes b(5) and P 450, glucuronyltransferase, galactosyltransferase, esterase, alkaline and acid phosphatases, 5'-nucleotidase, glucose 6-phosphatase, alkaline phosphodiesterase I, N-acetyl-beta-glucosaminidase, beta-glucuronidase, nucleoside diphosphatase, aldolase, fumarase, glutamine synthetase, protein, phospholipid, cholesterol, and RNA are described and justified when necessary.

MeSH Terms
Acid Phosphatase/analysis Alkaline Phosphatase/analysis Animals Autoanalysis Catalase/analysis Cell Fractionation Cytochrome Reductases/analysis Esterases/analysis Fructose-Bisphosphate Aldolase/analysis Glucose-6-Phosphatase/analysis Glucuronosyltransferase/analysis Glutamate-Ammonia Ligase/analysis Hexosyltransferases/analysis Liver/analysis,cytology Membranes/enzymology Microsomes, Liver/enzymology Monoamine Oxidase/analysis Nucleotidases/analysis Phospholipids/analysis Phosphoric Diester Hydrolases/analysis Phosphoric Monoester Hydrolases/analysis Proteins/analysis RNA/analysis Rats Subcellular Fractions/enzymology
Chemicals
Phospholipids Proteins RNA Catalase Monoamine Oxidase Cytochrome Reductases Hexosyltransferases Glucuronosyltransferase Esterases Nucleotidases Alkaline Phosphatase Acid Phosphatase Phosphoric Monoester Hydrolases Glucose-6-Phosphatase Phosphoric Diester Hydrolases Fructose-Bisphosphate Aldolase Glutamate-Ammonia Ligase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Beaufay H
Amar-Costesec A
Feytmans E
Thinès-Sempoux D
Wibo M
Robbi M
Berthet J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.61.1.188
Published
1974-04-00
Pages
188-200
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109252
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.61.1.188 PMC Full Text View journal details
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